Any feedback?
Literature summary for 2.3.1.38 extracted from
- Studies on the mechanism of fatty acid synthesis. XVII. Preparation and general properties of acetyl coenzyme A and malonyl coenzyme A-acyl carrier protein transacylases (1966), J. Biol. Chem., 241, 2326-2332.
General Stability
| General Stability | Organism |
|---|---|
| EDTA at 5 mM stabilize | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | - |
Escherichia coli |  |
| N-ethylmaleimide | - |
Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli E26 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.059 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -10°C, unpredictable instability on storage, sometimes total loss of activity occured immediately after thawing | Escherichia coli |
| 0°C, inactivation within several days | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + [acyl-carrier protein] | via acetyl-enzyme intermediate | Escherichia coli | CoA + acetyl-[acyl-carrier protein] | - |
r | |
| acetyl-CoA + [acyl-carrier protein] | via acetyl-enzyme intermediate | Escherichia coli E26 | CoA + acetyl-[acyl-carrier protein] | - |
r | |
| butyryl-CoA + [acyl-carrier protein] | 9.8% activity of that with acetyl-CoA | Escherichia coli | CoA + butyryl-[acyl-carrier protein] | - |
? | |
| butyryl-CoA + [acyl-carrier protein] | 9.8% activity of that with acetyl-CoA | Escherichia coli E26 | CoA + butyryl-[acyl-carrier protein] | - |
? | |
| hexanoyl-CoA + [acyl-carrier protein] | 4.6% activity of that with acetyl-CoA | Escherichia coli | CoA + hexanoyl-[acyl-carrier protein] | - |
? | |
| hexanoyl-CoA + [acyl-carrier protein] | 4.6% activity of that with acetyl-CoA | Escherichia coli E26 | CoA + hexanoyl-[acyl-carrier protein] | - |
? | |
| octanoyl-CoA + [acyl-carrier protein] | 10.3% activity of that with acetyl-CoA | Escherichia coli | CoA + octanoyl-[acyl-carrier protein] | - |
? | |
| octanoyl-CoA + [acyl-carrier protein] | 10.3% activity of that with acetyl-CoA | Escherichia coli E26 | CoA + octanoyl-[acyl-carrier protein] | - |
? | |
| propionyl-CoA + [acyl-carrier protein] | transacylation at 23.4% the rate of acetyl-CoA | Escherichia coli | CoA + propionyl-[acyl-carrier protein] | - |
? | |
| propionyl-CoA + [acyl-carrier protein] | transacylation at 23.4% the rate of acetyl-CoA | Escherichia coli E26 | CoA + propionyl-[acyl-carrier protein] | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 38 | - |
assay at | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
30% loss of activity after 40 min | Escherichia coli |
| 50 | - |
t1/2: 18 min | Escherichia coli |
| 60 | - |
t1/2: 9 min | Escherichia coli |
| 100 | - |
inactivation after 1 min | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.5 | about 60% of maximal activity at pH 5.0 and pH 8.5 | Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
