Literature summary for 2.3.1.35 extracted from

Iqbal, A.; Clifton, I.J.; Chowdhury, R.; Ivison, D.; Domene, C.; Schofield, C.J.
Structural and biochemical analyses reveal how ornithine acetyl transferase binds acidic and basic amino acid substrates (2011), Org. Biomol. Chem., 9, 6219-6225.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals of OAT2 in complex with L-Glu are generated. Optimization of crystallization conditions lead to a 2.7 A resolution structure for OAT2 acylated at Thr-181 and in complex with L-Glu (referred to as the acetyl-OAT2-glutamate complex)	Streptomyces clavuligerus

Protein Variants

Protein Variants	Comment	Organism
D150G	autoprocessing to alpha-,beta-subunits: 50% (compared to wild-type 100%), acetyl transferase activity (production of ornithine from N-alpha-acetyl-L-ornithine/L-Glu): 50% (wild-type 100%)	Streptomyces clavuligerus
DELTA1389	autoprocessing to alpha-,beta-subunits: 100% (compared to wild-type 100%), acetyl transferase activity (production of ornithine from N-alpha-acetyl-L-ornithine/L-Glu): 40% (wild-type 100%)	Streptomyces clavuligerus
E260A	autoprocessing to alpha-,beta-subunits: not determined (compared to wild-type 100%), acetyl transferase activity (production of ornithine from N-alpha-acetyl-L-ornithine/L-Glu): 40% (wild-type 100%)	Streptomyces clavuligerus
K170A	autoprocessing to alpha-,beta-subunits: 0% (compared to wild-type 100%), acetyl transferase activity (production of ornithine from N-alpha-acetyl-L-ornithine/L-Glu): 5% (wild-type 100%)	Streptomyces clavuligerus
T148A	autoprocessing to alpha-,beta-subunits: 0% (compared to wild-type 100%), acetyl transferase activity (production of ornithine from N-alpha-acetyl-L-ornithine/L-Glu): 2% (wild-type: 100%)	Streptomyces clavuligerus
T149A	autoprocessing to alpha-,beta-subunits: 80% (compared to wild-type 100%), acetyl transferase activity (production of ornithine from N-alpha-acetyl-L-ornithine/L-Glu): 10% (wild-type: 100%)	Streptomyces clavuligerus

Organism

Organism	UniProt	Comment	Textmining
Streptomyces clavuligerus	P0DJQ5	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-ornithine + N-acetyl-L-glutamate	-	Streptomyces clavuligerus	N2-acetyl-L-ornithine + L-glutamate	-	?

Synonyms

Synonyms	Comment	Organism
OAT2	-	Streptomyces clavuligerus
ornithine acetyl transferase	-	Streptomyces clavuligerus

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Release 2023.1 (January 2023)