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Literature summary for 2.3.1.35 extracted from
- Purification and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon (2005), FEBS J., 272, 5353-5364.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Citrullus lanatus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibited by citrulline or arginine at physiologically relevant high concentrations | Citrullus lanatus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.4 | - |
N2-Acetyl-L-ornithine | pH 7.0 | Citrullus lanatus |  |
| 17.8 | - |
L-glutamate | pH 7.0 | Citrullus lanatus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21300 | - |
2 * 21300 + 2 * 23500, calculated from sequence | Citrullus lanatus |
| 23500 | - |
2 * 21300 + 2 * 23500, calculated from sequence | Citrullus lanatus |
| 90000 | - |
gel filtration | Citrullus lanatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N2-acetyl-L-ornithine + L-glutamate | Citrullus lanatus | CLGAT can effectively participate in the biosynthesis of citrulline in leaves of during drought/strong-light stress | L-ornithine + N-acetyl-L-glutamate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrullus lanatus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the two subunits withj molecular masses of 21300 Da and 23500 Da are derived from a single precursor polypeptide, suggesting that the CLGAT precursor is cleaved autocatalytically at the conserved ATML motif. The first 26-amino acid sequence at the N-terminus of the precursor functions as a chloroplast transit peptide | Citrullus lanatus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Citrullus lanatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Citrullus lanatus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.7 | - |
above | Citrullus lanatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N2-acetyl-L-ornithine + L-glutamate | - |
Citrullus lanatus | L-ornithine + N-acetyl-L-glutamate | - |
? | |
| N2-acetyl-L-ornithine + L-glutamate | CLGAT can effectively participate in the biosynthesis of citrulline in leaves of during drought/strong-light stress | Citrullus lanatus | L-ornithine + N-acetyl-L-glutamate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 2 * 21300 + 2 * 23500, calculated from sequence | Citrullus lanatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CLGAT | - |
Citrullus lanatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Citrullus lanatus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 80 | 40°C: about 40% of maximal activity, 80°C: about 45% of maximal activity | Citrullus lanatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
stable up to | Citrullus lanatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Citrullus lanatus |
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Release 2023.1 (January 2023)
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