Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | cysteine synthase bienzyme complex | Arabidopsis thaliana | |
0.91 | - |
acetyl-CoA | free recombinant enzyme | Arabidopsis thaliana | |
2.84 | - |
L-serine | free recombinant enzyme | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
2 * 34000, serine acetyltransferase + 2 * 36000, O-acetylserine (thiol) lyase, forming the cysteine synthase complex, SDS-PAGE | Arabidopsis thaliana |
36000 | - |
2 * 34000, serine acetyltransferase + 2 * 36000, O-acetylserine (thiol) lyase, forming the cysteine synthase complex, SDS-PAGE | Arabidopsis thaliana |
300000 | - |
cysteine synthase bienzyme complex, gel filtration | Arabidopsis thaliana |
2000000 | - |
enzyme aggregate, gel filtration | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | Arabidopsis thaliana | regulatory role in cysteine biosynthesis | CoA + O-acetyl-L-serine | - |
ir | |
additional information | Arabidopsis thaliana | cysteine synthase is a bienzyme complex build of serine acetyltransferase and O-acetylserine (thiol) lyase, no channeling of substrate within the complex | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from E. coli | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.4 | - |
recombinant protein, crude extract from E. coli | Arabidopsis thaliana |
70 | - |
purified recombinant enzyme | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | - |
Arabidopsis thaliana | CoA + O-acetyl-L-serine | - |
ir | |
acetyl-CoA + L-serine | regulatory role in cysteine biosynthesis | Arabidopsis thaliana | CoA + O-acetyl-L-serine | - |
ir | |
additional information | cysteine synthase is a bienzyme complex build of serine acetyltransferase and O-acetylserine (thiol) lyase, no channeling of substrate within the complex | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 34000, serine acetyltransferase + 2 * 36000, O-acetylserine (thiol) lyase, forming the cysteine synthase complex, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
More | serine acetyltransferase and O-acetylserine (thiol) lyase form the cysteine synthase complex, consisting of 2 of each enzymes as a tetramer | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | 4 mol per mol of cysteine synthase complex | Arabidopsis thaliana |