Cloned (Comment) | Organism |
---|---|
recombinant expresssion in Escherichia coli | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of the exchange of pro-R hydrogen of glycine with protons in the medium by the enzyme | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43117.15, recombinant enzyme, mass spectrometry, x * 43000, recombinant enzyme, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + glycine | Escherichia coli | condensation reaction | CoA + L-2-amino-3-oxobutanoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, followed by gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + glycine | condensation reaction | Escherichia coli | CoA + L-2-amino-3-oxobutanoate | - |
? | |
additional information | the enzyme catalyzes the exchange of pro-R hydrogen of glycine with protons in the medium | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43117.15, recombinant enzyme, mass spectrometry, x * 43000, recombinant enzyme, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
2-amino-3-ketobutyrate CoA ligase | - |
Escherichia coli |
KBL | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
evolution | 2-amino-3-ketobutyrate CoA ligase of Escherichia coli is a member of the alpha-oxoamine synthase family | Escherichia coli |