Cloned (Comment) | Organism |
---|---|
using the baculovirus system | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(24R)-ethylcholest-5-ene-3,7-diol | - |
Homo sapiens | |
3beta-hydroxylup-20(29)-en-28-oic acid | - |
Homo sapiens | |
5alpha,8alpha-epidioxy-24(R)-methylcholesta-6,22-diene-3beta-ol | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + cholesterol | Homo sapiens | - |
CoA + cholesterol ester | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
microsomal fractions of Hi5 cells containing baculovirally expressed hACAT-1 are used as sources of the enzyme | Homo sapiens |
microsomal fractions of Hi5 cells containing baculovirally expressed hACAT-2 are used as sources of the enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + cholesterol | - |
Homo sapiens | CoA + cholesterol ester | - |
? | |
oleoyl-CoA + cholesterol | activity assay | Homo sapiens | CoA + cholesteryl oleate | - |
? | |
oleoyl-CoA + cholesterol | activity assay | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acyl-CoA:cholesterol acyltransferase | - |
Homo sapiens |
hACAT-1 | - |
Homo sapiens |
hACAT-2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
activity assay | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0115 | - |
- |
Homo sapiens | 3beta-hydroxylup-20(29)-en-28-oic acid | |
0.025 | - |
- |
Homo sapiens | (24R)-ethylcholest-5-ene-3,7-diol | |
0.0339 | - |
- |
Homo sapiens | 3beta-hydroxylup-20(29)-en-28-oic acid | |
0.0387 | - |
- |
Homo sapiens | 5alpha,8alpha-epidioxy-24(R)-methylcholesta-6,22-diene-3beta-ol | |
0.102 | - |
- |
Homo sapiens | (24R)-ethylcholest-5-ene-3,7-diol | |
0.2 | - |
- |
Homo sapiens | 5alpha,8alpha-epidioxy-24(R)-methylcholesta-6,22-diene-3beta-ol |