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Literature summary for 2.3.1.26 extracted from
- Rat liver acyl-coenzyme A:cholesterol acyltransferase: its regulation in vivo and some of its properties in vitro (1980), J. Lipid Res., 21, 930-941.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 25-hydroxycholesterol | activates | Rattus norvegicus |  |
| cholate | increase of activity in cholate-fed animals | Rattus norvegicus | |
| cholesterol | increase of activity in cholesterol-fed animals | Rattus norvegicus | |
| Mevalonolactone | intragastric administration, increasing sterol biosynthesis and increasing enzyme activity | Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetone | 30-50% inhibition at a final concentration of 1% v/v, inhibition prevented by the addition of cholesterol to the solvent | Rattus norvegicus | |
| butanol | 30-50% inhibition at a final concentration of 1% v/v, inhibition prevented by the addition of cholesterol to the solvent | Rattus norvegicus | |
| Detergents | above their critical micelle concentration, e.g. cholate, deoxycholate, cetyltriethylammonium bromide, Triton X-100, Tween 20, Tween 80, Nonidet P40 | Rattus norvegicus | |
| Digitonin | - |
Rattus norvegicus | |
| ethanol | 30-50% inhibition at a final concentration of 1% v/v, inhibition prevented by the addition of cholesterol to the solvent | Rattus norvegicus | |
| Filipin | - |
Rattus norvegicus | |
| oleoyl-CoA | inhibits at high concentrations, the inhibition is prevented by serum albumin | Rattus norvegicus | |
| organic solvents | - |
Rattus norvegicus | |
| progesterone | - |
Rattus norvegicus | |
| SC-31769 | 20-oxa analog of 7-ketosterol | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | little activity | Rattus norvegicus | 5829 | - |
| endoplasmic reticulum | rough, membranes | Rattus norvegicus | 5783 | - |
| microsome | intrinsic membrane enzyme, highest activity | Rattus norvegicus | - |
- |
| mitochondrion | little activity | Rattus norvegicus | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| long-chain fatty acyl-CoA + cholesterol | Rattus norvegicus | the enzyme plays an important role in maintenance of hepatic cholesterol homeostasis | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | Rattus norvegicus male Sprague-Dawley | the enzyme plays an important role in maintenance of hepatic cholesterol homeostasis | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| additional information | the use of organic solvents or strong detergents for the suspension of cholesterol must be avoided because of their affect on the enzyme | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male Sprague-Dawley strain | - |
| Rattus norvegicus male Sprague-Dawley | - |
male Sprague-Dawley strain | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
overview: effect of fasting, feeding with cholesterol or cholate, atherogenic diet administration of Triton WR 1339 and administration of mevanolactone, evidence for a circadian rhythm | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| frozen, stable for up to 2 weeks | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + cholesterol | - |
Rattus norvegicus | CoA + cholesterol ester | - |
? | |
| acyl-CoA + cholesterol | - |
Rattus norvegicus male Sprague-Dawley | CoA + cholesterol ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | the enzyme plays an important role in maintenance of hepatic cholesterol homeostasis | Rattus norvegicus | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | the enzyme plays an important role in maintenance of hepatic cholesterol homeostasis | Rattus norvegicus male Sprague-Dawley | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| oleoyl-CoA + cholesterol | - |
Rattus norvegicus | CoA + cholesteryl oleate | - |
? | |
| oleoyl-CoA + cholesterol | - |
Rattus norvegicus male Sprague-Dawley | CoA + cholesteryl oleate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
10 min, complete loss of activity | Rattus norvegicus |
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