Protein Variants | Comment | Organism |
---|---|---|
S341A | mutation in potential phosphorylation site, about 45% of wild-type activity, substrate tetracosanoyl-CoA | Mus musculus |
S349A | mutation in potential phosphorylation site, about 90% of wild-type activity, substrate tetracosanoyl-CoA | Mus musculus |
T346A | mutation in potential phosphorylation site, about 80% of wild-type activity, substrate tetracosanoyl-CoA | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00107 | - |
sphingosine | absence of lambda protein phosphatase, pH 7.4, 37°C | Mus musculus | |
0.0077 | - |
sphingosine | presence of lambda protein phosphatase, pH 7.4, 37°C | Mus musculus | |
0.0546 | - |
tetracosanoyl-CoA | presence of lambda protein phosphatase, pH 7.4, 37°C | Mus musculus | |
0.0629 | - |
tetracosanoyl-CoA | absence of lambda protein phosphatase, pH 7.4, 37°C | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8IU89 | isoform CerS3 | - |
Homo sapiens | Q8N5B7 | isoform CerS5 | - |
Homo sapiens | Q96G23 | isoform CerS2 | - |
Homo sapiens | Q9HA82 | isoform CerS4 | - |
Homo sapiens | Q9NTG7 | isoform CerS6 | - |
Mus musculus | Q924Z4 | isoform CerS2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Mus musculus |
glycoprotein | - |
Homo sapiens |
no glycoprotein | - |
Homo sapiens |
phosphoprotein | isoform Cers2 is phosphorylated at the cytoplasmic C-terminal region. Treatment of cells with the casein kinase CK2-specific inhibitor CX-4945 lowers the phosphorylation level. Phosphorylation of CERS2 is important for its catalytic activity, acting mainly by increasing its Vmax value | Homo sapiens |
phosphoprotein | isoform Cers2 is phosphorylated at the cytoplasmic C-terminalregion | Mus musculus |
phosphoprotein | isoform Cers3 is phosphorylated at the cytoplasmic C-terminal region. Treatment of cells with the casein kinase CK2-specific inhibitor CX-4945 does not lower the phosphorylation level. Treatment with lambda protein phosphatase reduces the activity by 28% | Homo sapiens |
phosphoprotein | isoform Cers4 is phosphorylated at the cytoplasmic C-terminal region. Treatment with lambda protein phosphatase reduces the activity by 52% | Homo sapiens |
phosphoprotein | isoform Cers5 is phosphorylated at the cytoplasmic C-terminal region. Treatment with lambda protein phosphatase reduces the activity by 27% | Homo sapiens |
phosphoprotein | isoform Cers6 is phosphorylated by serine kinase CK2 at the cytoplasmic C-terminal region. Treatment with lambda protein phosphatase reduces the activity by 10% | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
docosanoyl-CoA + sphingosine | - |
Homo sapiens | CoA + N-docosanoylsphingosine | - |
? | |
eicosanoyl-CoA + sphingosine | - |
Homo sapiens | CoA + N-eicosanoylsphingosine | - |
? | |
hexacosanoyl-CoA + sphingosine | - |
Homo sapiens | CoA + N-hexacosanoylsphingosine | - |
? | |
additional information | expression of CERS3 increases the levels of C18-C22-ceramides, whereas it reduces the levels of C16:0-ceramide and C24-ceramides | Homo sapiens | ? | - |
- |
|
additional information | expression of CERS3 increases the levels of C26:0-ceramide as well as those of C18:0-ceramide, C20:0-ceramide, and C24:0-ceramide, whereas it reduces the level of C16:0-ceramide | Homo sapiens | ? | - |
- |
|
additional information | expression of CERS5 increases the level of C16:0-ceramide and decreases levels of C18:0-ceramide, C22:0-ceramide, and C24-ceramides | Homo sapiens | ? | - |
- |
|
palmitoyl-CoA + sphingosine | - |
Homo sapiens | CoA + N-palmitoylsphingosine | - |
? | |
palmitoyl-CoA + sphingosine | - |
Homo sapiens | CoA + palmitoylsphingosine | - |
? | |
stearoyl-CoA + sphingosine | - |
Homo sapiens | CoA + N-stearoylsphingosine | - |
? | |
tetracosanoyl-CoA + sphingosine | - |
Mus musculus | CoA + N-tetracosanoylsphingosine | - |
? | |
tetracosanoyl-CoA + sphingosine | - |
Homo sapiens | CoA + N-tetracosanoylsphingosine | - |
? |