Cloned (Comment) | Organism |
---|---|
expressed in COS-7 cell | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F352C | naturally occuring mutation | Homo sapiens |
F352C/V368I | double mutant with naturally occuring mutations | Homo sapiens |
F352C/V368I/V605L | triple mutant with naturally occuring mutations | Homo sapiens |
M647V | naturally occuring mutation | Homo sapiens |
P504L | naturally occuring mutation | Homo sapiens |
P504L/V605L | double mutant with naturally occuring mutations | Homo sapiens |
V368I | naturally occuring mutation | Homo sapiens |
V605L | naturally occuring mutation | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
L-carnitine | wild-type protein, COS-7 cell lysate | Homo sapiens | |
0.13 | - |
L-carnitine | V368I mutant protein, COS-7 cell lysate | Homo sapiens | |
0.14 | - |
L-carnitine | M647V mutant protein, COS-7 cell lysate | Homo sapiens | |
0.35 | - |
L-carnitine | P504L mutant protein, COS-7 cell lysate | Homo sapiens | |
0.35 | - |
L-carnitine | V605L mutant protein, COS-7 cell lysate | Homo sapiens | |
0.36 | - |
L-carnitine | F352C mutant protein, COS-7 cell lysate | Homo sapiens | |
0.37 | - |
L-carnitine | F352C/V368I mutant protein, COS-7 cell lysate | Homo sapiens | |
0.37 | - |
L-carnitine | wild-type protein and P504L/V605L mutant protein, COS-7 cell lysate (doubly transfected) | Homo sapiens | |
0.38 | - |
L-carnitine | P504L/V605L mutant protein, COS-7 cell lysate | Homo sapiens | |
0.52 | - |
L-carnitine | F352C/V368I/V605L mutant protein, COS-7 cell lysate | Homo sapiens | |
0.53 | - |
L-carnitine | wild-type protein and F352C/V368I/V605L mutant protein, COS-7 cell lysate (doubly transfected) | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | - |
Homo sapiens | 5743 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P23786 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00062 | - |
F352C/V368I/V605L mutant protein, COS-7 cell lysate | Homo sapiens |
0.00063 | - |
wild-type protein and F352C/V368I/V605L mutant protein, COS-7 cell lysate (doubly transfected) | Homo sapiens |
0.00071 | - |
P504L/V605L mutant protein, COS-7 cell lysate | Homo sapiens |
0.00072 | - |
F352C/V368I mutant protein, COS-7 cell lysate | Homo sapiens |
0.00072 | - |
wild-type protein and P504L/V605L mutant protein, COS-7 cell lysate (doubly transfected) | Homo sapiens |
0.00073 | - |
F352C mutant protein, COS-7 cell lysate | Homo sapiens |
0.00076 | - |
P504L mutant protein, COS-7 cell lysate | Homo sapiens |
0.00078 | - |
V605L mutant protein, COS-7 cell lysate | Homo sapiens |
0.00081 | - |
M647V mutant protein, COS-7 cell lysate | Homo sapiens |
0.00085 | - |
V368I mutant protein, COS-7 cell lysate | Homo sapiens |
0.00087 | - |
wild-type protein, COS-7 cell lysate | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-carnitine + palmitoyl-CoA | - |
Homo sapiens | L-palmitoylcarnitine + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carnitine palmitoyltransferase II | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
mutant proteins V605L, F352C, and P504L: enzyme activities of transfected cells are significantly reduced to 59.5 to 43.6% during incubation for 2 hours at 41°C, mutant protein F352C/V368I/V605L: enzyme activity of transfected cells is reduced to 19.4% during incubation for 2 hours at 41°C | Homo sapiens |