Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Ovis aries |
Protein Variants | Comment | Organism |
---|---|---|
additional information | chimaera in which the distinctive N-terminal segmant of ovine m-BPT 1 is replaced with that from rat M-CPT 1. The ovine N-terminal segment influences the kinetics of the enzyme for both its substrates, such that the Km for palmitoyl-CoA is decreased and that for carnitine is increased for the chimera, relative to the parental enzyme M-CPT 1 from Ovis aries | Ovis aries |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
malonyl-CoA | IC50 for wild-type L-CPT-1 is 0.0298 mM; IC50 for wild-type M-CPT-1 is 0.010 mM | Ovis aries |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
palmitoyl-CoA | chimaera in which the distinctive N-terminal segment of ovine m-BPT 1 is replaced with that from rat M-CPT 1 | Ovis aries | |
0.0374 | - |
palmitoyl-CoA | wild-type L-CPT1 | Ovis aries | |
0.0676 | - |
palmitoyl-CoA | wild-type M-CPT1 | Ovis aries | |
0.215 | - |
L-carnitine | wild-type L-CPT 1 | Ovis aries | |
0.56 | - |
L-carnitine | wild-type M-CPT 1 | Ovis aries | |
0.785 | - |
L-carnitine | chimaera in which the distinctive N-terminal segment of ovine m-BPT 1 is replaced with that from rat M-CPT 1 | Ovis aries |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ovis aries | Q7YQR7 | - |
- |
Ovis aries | Q8SPI7 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adipose tissue | - |
Ovis aries | - |
brain | - |
Ovis aries | - |
brain | high level of mRNA | Ovis aries | - |
heart | - |
Ovis aries | - |
heart | high level of mRNA | Ovis aries | - |
kidney | - |
Ovis aries | - |
kidney | high level of mRNA | Ovis aries | - |
liver | - |
Ovis aries | - |
liver | high level of mRNA | Ovis aries | - |
lung | - |
Ovis aries | - |
mammary gland | lactating, high level of mRNA | Ovis aries | - |
mammary gland | non-lactating, high level of mRNA | Ovis aries | - |
additional information | no activity in spleen | Ovis aries | - |
pancreas | - |
Ovis aries | - |
skeletal muscle | - |
Ovis aries | - |
skeletal muscle | high level of mRNA | Ovis aries | - |
spleen | - |
Ovis aries | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + L-carnitine | - |
Ovis aries | CoA + L-palmitoylcarnitine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CPT1A | - |
Ovis aries |
CPT1B | - |
Ovis aries |
L-CPT 1 | - |
Ovis aries |
M-CPT 1 | - |
Ovis aries |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.01 | - |
IC50 for wild-type M-CPT-1 is 0.010 mM | Ovis aries | malonyl-CoA | |
0.0298 | - |
IC50 for wild-type L-CPT-1 is 0.0298 mM | Ovis aries | malonyl-CoA |