Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.21 extracted from

  • Colquhoun, A.
    Gamma-linolenic acid alters the composition of mitochondrial membrane subfractions, decreases outer mitochondrial membrane binding of hexokinase and alters carnitine palmitoyltransferase I properties in the Walker 256 rat tumour (2002), Biochim. Biophys. Acta, 1583, 74-84.
    View publication on PubMed
Search for more literature for 2.3.1.21

Application

Application Comment Organism
pharmacology CPT I, model enzyme for studies of fatty acid-induced apoptosis in cancer therapy Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
gamma-linolenic acid inhibits CPT I in vivo and reduces malonyl-CoA sensitivity, decreases affinity for 16:0 acyl-CoA substrate Rattus norvegicus
malonyl-CoA CPT I, sensitivity is reduced in vivo during gamma-linolenic acid treatment; membrane or micelle composition and properties influence the sensitivity of CPT I to inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 palmitoyl-CoA CPT I Rattus norvegicus
0.017
-
 palmitoyl-CoA CPT I after gamma-linoleic acid treatment Rattus norvegicus
0.092
-
 L-carnitine CPT I Rattus norvegicus
0.098
-
 L-carnitine CPT I after gamma-linoleic acid treatment Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial outer membrane CPT I is located in the outer membrane Rattus norvegicus 5741
-
mitochondrion
-
 Rattus norvegicus 5739
-
additional information composition of the outer mitochondrial membrane is important for functionality and activity of CPT I Rattus norvegicus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + L-carnitine Rattus norvegicus enzyme is involved in apoptosis induction CoA + L-acylcarnitine
-
 ?
additional information Rattus norvegicus gamma-linolenic acid diet leads to several physiological effects during cancer therapy, e.g. reduction of mitochondrial metabolic activity, modification of mitochondrial outer membrane composition, increase in lipid peroxidation, cytoplasmic accumulation of acyl-CoA, reduction of CPT I activity, and malonyl-CoA sensitivity ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 enzyme form CPT I
-
Rattus norvegicus
-
 Walker 256 tumour rat
-

Source Tissue

Source Tissue Comment Organism Textmining
Walker carcinoma 256 cell CPT I Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0014
-
 CPT I after gamma-linoleic acid treatment Rattus norvegicus
0.0036
-
 CPT I Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + L-carnitine activity with different substrates of wild-type CPT I with and without gamma-linoleic acid treatment Rattus norvegicus CoA + L-acylcarnitine
-
 ?
acyl-CoA + L-carnitine enzyme is involved in apoptosis induction Rattus norvegicus CoA + L-acylcarnitine
-
 ?
additional information gamma-linolenic acid diet leads to several physiological effects during cancer therapy, e.g. reduction of mitochondrial metabolic activity, modification of mitochondrial outer membrane composition, increase in lipid peroxidation, cytoplasmic accumulation of acyl-CoA, reduction of CPT I activity, and malonyl-CoA sensitivity Rattus norvegicus ?
-
 ?
palmitoyl-CoA + L-carnitine
-
 Rattus norvegicus CoA + L-palmitoylcarnitine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus