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Literature summary for 2.3.1.192 extracted from

  • Nandi, D.L.; Lucas, S.V.; Webster, L.T.
    Benzoyl-coenzyme A:glycine N-acyltransferase and phenylacetyl-coenzyme A:glycine N-acyltransferase from bovine liver mitochondria. Purification and characterization (1979), J. Biol. Chem., 254, 7230-7237.
    View publication on PubMed
Search for more literature for 2.3.1.192

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate) phenylacetyl-CoA partially protects phenylacetyltransferase against 5,5'-dithiobis(2-nitrobenzoate) inactivation Bos taurus
acylglycine noncompetitive Bos taurus
benzoyl-CoA
-
 Bos taurus
butyryl-CoA
-
 Bos taurus
CoA noncompetitve Bos taurus
Ni2+ 2 mM, 28% residual activity Bos taurus
p-chloromercuribenzoate
-
 Bos taurus
Zn2+ 1 mM, 46% residual activity Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information addition of monovalent cations is required Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 gel filtration Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Reaction

Reaction Comment Organism Reaction ID
phenylacetyl-CoA + glycine = CoA + phenylacetylglycine sequential reaction mechanism in which the acyl-CoA substrate adds to the enzyme first, glycine adds before CoA leaves, and the peptide product dissociates last Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.051
-
 pH not specified in the publication, temperature not specified in the publication Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
indoleacetyl-CoA + glycine
-
 Bos taurus N-indoleacetylglycine + CoA
-
 ?
phenylacetyl-CoA + glycine glycine is the preferred acceptor Bos taurus phenylacetylglycine + CoA
-
 ?
phenylacetyl-CoA + L-asparagine stereospecific for the L-isomer of asparagine Bos taurus ? + CoA
-
 ?
phenylacetyl-CoA + L-glutamine stereospecific for the L-isomer of glutamine Bos taurus ? + CoA
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 33000, SDS-PAGE Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4 8.6
-
 Bos taurus