Literature summary for 2.3.1.181 extracted from

Fujiwara, K.; Hosaka, H.; Matsuda, M.; Okamura-Ikeda, K.; Motokawa, Y.; Suzuki, M.; Nakagawa, A.; Taniguchi, H.
Crystal structure of bovine lipoyltransferase in complex with lipoyl-AMP (2007), J. Mol. Biol., 371, 222-234.

Search for more literature for 2.3.1.181

Cloned(Commentary)

Cloned (Comment)	Organism
for expression in Escherichia coli BL21DE3 pLysS cells	Bos taurus

Crystallization (Commentary)

Crystallization (Comment)	Organism
the crystal structure of lipoyltransferase in complex with lipoyl-AMP is determined at a resolution of 2.1 A	Bos taurus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Bos taurus	5739	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
octanoyl-[acyl-carrier protein] + protein	Bos taurus	-	protein N6-(octanoyl)lysine + [acyl-carrier protein]	-	?

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	O46419	-	-

Purification (Commentary)

Purification (Comment)	Organism
hydroxylapatite and DEAE-Sepharose columns are used for the purification of the recombinant protein and for the purification of lipoyltransferase from bovine liver mitochondria	Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
octanoyl-[acyl-carrier protein] + protein	-	Bos taurus	protein N6-(octanoyl)lysine + [acyl-carrier protein]	-	?

Synonyms

Synonyms	Comment	Organism
bLT	-	Bos taurus
lipoyl(octanoyl) transferase	-	Bos taurus
lipoyltransferase	-	Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay of lipoylation	Bos taurus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	assay of lipoylation	Bos taurus

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Release 2023.1 (January 2023)