Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Acinetobacter baumannii |
Crystallization (Comment) | Organism |
---|---|
apoenzyme or mutant enzymes C155A, C264A and C155A/C264A in complex with octanoyl-CoA, using PEG3350 as a precipitant and Tris HCl buffer (pH 7.0-8.0) and 0.1 M calcium acetate | Acinetobacter baumannii |
Protein Variants | Comment | Organism |
---|---|---|
F56W | the mutation does not show a drastic change in reactivity towards octanoyl-CoA even though the band of the variant is reduced approximately by 40% compared to the wild type | Acinetobacter baumannii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + a malonyl-[acyl-carrier protein] | Acinetobacter baumannii | - |
an acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baumannii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sepharose column chromatography | Acinetobacter baumannii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + a malonyl-[acyl-carrier protein] | - |
Acinetobacter baumannii | an acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
octanoyl-CoA + malonyl-[acyl-carrier protein] | - |
Acinetobacter baumannii | 3-oxo-decanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-ketoacyl-acyl carrier protein synthase III | - |
Acinetobacter baumannii |
KAS III | - |
Acinetobacter baumannii |