Cloned (Comment) | Organism |
---|---|
gene fabH or Xoo4209, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Xanthomonas oryzae pv. oryzae |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged KASIII, by sitting-drop vapour-diffusion method, 4 mg/ml proteinin 25 mM Tris-HCl, pH 7.5, 20% glycerol, 3 mM 2-mercaptoethanol, 1 M NaCl is mixed with precipitatnt solution containing 0.1 M HEPES, pH 7.2, 30% PEG 6000, 5% 2-methyl-2,4-pentanediol and 3% D-galactose, X-ray diffraction structure determination and analysis at 2.05 A resolution | Xanthomonas oryzae pv. oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, recombinant His-tagged enzyme, SDS-PAGE | Xanthomonas oryzae pv. oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas oryzae pv. oryzae | - |
gene fabH or Xoo4209 | - |
Xanthomonas oryzae pv. oryzae Xoo KACC10331 | - |
gene fabH or Xoo4209 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography | Xanthomonas oryzae pv. oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | structure-function relationship, overview | Xanthomonas oryzae pv. oryzae | ? | - |
? | |
additional information | structure-function relationship, overview | Xanthomonas oryzae pv. oryzae Xoo KACC10331 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, recombinant His-tagged enzyme, SDS-PAGE | Xanthomonas oryzae pv. oryzae |
More | structure-function relationship, overview | Xanthomonas oryzae pv. oryzae |
Synonyms | Comment | Organism |
---|---|---|
beta-ketoacyl-ACP synthase III | - |
Xanthomonas oryzae pv. oryzae |
FabH | - |
Xanthomonas oryzae pv. oryzae |
General Information | Comment | Organism |
---|---|---|
physiological function | FabH is an important enzyme in the elongation steps of fatty-acid biosynthesis | Xanthomonas oryzae pv. oryzae |