Cloned (Comment) | Organism |
---|---|
His-tagged protein expressed in Escherichia coli pLysS | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
R206G | R206 impairs the binding of CoA | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0061 | - |
malonyl-phosphopantetheine-14-mer | wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C | Escherichia coli | |
0.0062 | - |
malonyl-phosphopantetheine-16-mer | wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C | Escherichia coli | |
0.0082 | - |
malonyl-ACP | wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C | Escherichia coli | |
0.0237 | - |
malonyl-phosphopantetheine-8-mer | wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C | Escherichia coli | |
0.0283 | - |
malonyl-CoA | R206G mutant protein, donor lauroyl-CoA, 0.1 mM lauroyl-CoA, pH 6.5, 25°C | Escherichia coli | |
0.0537 | - |
lauroyl-CoA | wild type protein, acceptor malonyl-CoA, 0.5 mM malonyl-CoA, pH 6.5, 25°C | Escherichia coli | |
0.51 | - |
malonyl-CoA | wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C | Escherichia coli | |
0.59 | - |
malonyl-phosphopantetheine | wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+) | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-ACP + lauroyl-ACP | - |
Escherichia coli | ? | - |
? | |
malonyl-CoA + lauroyl-CoA | - |
Escherichia coli | ? | - |
? | |
malonyl-phosphopantetheine + lauroyl-CoA | - |
Escherichia coli | ? | - |
? | |
malonyl-phosphopantetheine-14-mer + lauroyl-CoA | - |
Escherichia coli | ? | - |
? | |
malonyl-phosphopantetheine-16-mer + lauroyl-CoA | - |
Escherichia coli | ? | - |
? | |
malonyl-phosphopantetheine-8-mer + lauroyl-CoA | - |
Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-ketoacyl-ACP synthase II | - |
Escherichia coli |
FabF | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
malonyl-phosphopantetheine-8-mer | wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C | Escherichia coli | |
0.022 | - |
malonyl-CoA | R206G mutant protein, donor lauroyl-CoA, 0.1 mM lauroyl-CoA, pH 6.5, 25°C | Escherichia coli | |
0.022 | - |
malonyl-phosphopantetheine-16-mer | wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C | Escherichia coli | |
0.022 | - |
malonyl-phosphopantetheine | wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C | Escherichia coli | |
0.023 | - |
malonyl-phosphopantetheine-14-mer | wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C | Escherichia coli | |
0.029 | - |
malonyl-ACP | wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C | Escherichia coli | |
0.042 | - |
malonyl-CoA | wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C | Escherichia coli | |
0.047 | - |
lauroyl-CoA | wild type protein, acceptor malonyl-CoA, 0.5 mM malonyl-CoA, pH 6.5, 25°C | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
malonyl-phosphopantetheine | wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C | Escherichia coli | |
0.083 | - |
malonyl-CoA | wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C | Escherichia coli | |
0.083 | - |
malonyl-phosphopantetheine-8-mer | wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C | Escherichia coli | |
0.83 | - |
malonyl-CoA | R206G mutant protein, donor lauroyl-CoA, 0.1 mM lauroyl-CoA, pH 6.5, 25°C | Escherichia coli | |
1 | - |
lauroyl-CoA | wild type protein, acceptor malonyl-CoA, 0.5 mM malonyl-CoA, pH 6.5, 25°C | Escherichia coli | |
3.8 | - |
malonyl-ACP | wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C | Escherichia coli | |
3.8 | - |
malonyl-phosphopantetheine-16-mer | wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C | Escherichia coli | |
4.2 | - |
malonyl-phosphopantetheine-14-mer | wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C | Escherichia coli |