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Literature summary for 2.3.1.179 extracted from

  • Borgaro, J.; Chang, A.; MacHutta, C.; Zhang, X.; Tonge, P.
    Substrate recognition by beta-ketoacyl-ACP synthases (2011), Biochemistry, 50, 10678-10686.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged protein expressed in Escherichia coli pLysS Escherichia coli

Protein Variants

Protein Variants Comment Organism
R206G R206 impairs the binding of CoA Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0061
-
malonyl-phosphopantetheine-14-mer wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
0.0062
-
malonyl-phosphopantetheine-16-mer wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
0.0082
-
malonyl-ACP wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
0.0237
-
malonyl-phosphopantetheine-8-mer wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
0.0283
-
malonyl-CoA R206G mutant protein, donor lauroyl-CoA, 0.1 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
0.0537
-
lauroyl-CoA wild type protein, acceptor malonyl-CoA, 0.5 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
0.51
-
malonyl-CoA wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
0.59
-
malonyl-phosphopantetheine wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Ni2+) Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-ACP + lauroyl-ACP
-
Escherichia coli ?
-
?
malonyl-CoA + lauroyl-CoA
-
Escherichia coli ?
-
?
malonyl-phosphopantetheine + lauroyl-CoA
-
Escherichia coli ?
-
?
malonyl-phosphopantetheine-14-mer + lauroyl-CoA
-
Escherichia coli ?
-
?
malonyl-phosphopantetheine-16-mer + lauroyl-CoA
-
Escherichia coli ?
-
?
malonyl-phosphopantetheine-8-mer + lauroyl-CoA
-
Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
beta-ketoacyl-ACP synthase II
-
Escherichia coli
FabF
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.018
-
malonyl-phosphopantetheine-8-mer wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
0.022
-
malonyl-CoA R206G mutant protein, donor lauroyl-CoA, 0.1 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
0.022
-
malonyl-phosphopantetheine-16-mer wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
0.022
-
malonyl-phosphopantetheine wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
0.023
-
malonyl-phosphopantetheine-14-mer wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
0.029
-
malonyl-ACP wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
0.042
-
malonyl-CoA wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
0.047
-
lauroyl-CoA wild type protein, acceptor malonyl-CoA, 0.5 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.037
-
malonyl-phosphopantetheine wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
0.083
-
malonyl-CoA wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
0.083
-
malonyl-phosphopantetheine-8-mer wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
0.83
-
malonyl-CoA R206G mutant protein, donor lauroyl-CoA, 0.1 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
1
-
lauroyl-CoA wild type protein, acceptor malonyl-CoA, 0.5 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
3.8
-
malonyl-ACP wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
3.8
-
malonyl-phosphopantetheine-16-mer wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
4.2
-
malonyl-phosphopantetheine-14-mer wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli