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Literature summary for 2.3.1.179 extracted from

  • Wang, J.; Kodali, S.; Lee, S.H.; Galgoci, A.; Painter, R.; Dorso, K.; Racine, F.; Motyl, M.; Hernandez, L.; Tinney, E.; Colletti, S.L.; Herath, K.; Cummings, R.; Salazar, O.; Gonzalez, I.; Basilio, A.; Vicente, F.; Genilloud, O.; Pelaez, F.; Jayasuriya, H.; Young, K.; Cully, D.F.; Singh, S.B.
    Discovery of platencin, a dual FabF and FabH inhibitor with in vivo antibiotic properties (2007), Proc. Natl. Acad. Sci. USA, 104, 7612-7616.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
drug development the enzyme is a target for antibacterial drugs Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
additional information in vivo inhibition assays Staphylococcus aureus
platencin exhibits a broad-spectrum Gram-positive antibacterial activity through inhibition of fatty acid biosynthesis, targets the two essential proteins, beta-ketoacyl-[acyl carrier protein] synthase II and III, i.e. FabF and FabH, FabF IC50: 113 nM, overview Staphylococcus aureus
platensimycin from a strain of Streptomyces platensis MA7339, specifically targets FabF, IC50: 290 nM, exhibits Gram-positive antibacterial activity Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-
Staphylococcus aureus
-
methicillin-resistant and vancomycin-resistant strains
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
development of an elongation assay with FabF and FabH, EC 2.3.1.41 Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information FabF produces C14 long-chain beta-ketoacyl-ACP Staphylococcus aureus ?
-
?

Synonyms

Synonyms Comment Organism
beta-ketoacyl-[acyl carrier protein (ACP)] synthase II
-
Staphylococcus aureus
beta-ketoacyl-[acyl-carrier protein (ACP)] synthase II
-
Staphylococcus aureus
FabF
-
Staphylococcus aureus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Staphylococcus aureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Staphylococcus aureus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
IC50 for platencin is 0.00195 ng/ml, IC50 for platensimycin is 0.00013 ng/ml Staphylococcus aureus additional information
0.000113
-
exhibits a broad-spectrum Gram-positive antibacterial activity through inhibition of fatty acid biosynthesis, targets the two essential proteins, beta-ketoacyl-[acyl carrier protein] synthase II and III, i.e. FabF and FabH, FabF IC50: 113 nM, overview Staphylococcus aureus platencin
0.00029
-
from a strain of Streptomyces platensis MA7339, specifically targets FabF, IC50: 290 nM, exhibits Gram-positive antibacterial activity Staphylococcus aureus platensimycin