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Literature summary for 2.3.1.179 extracted from

  • Huang, W.; Jia, J.; Edwards, P.; Dehesh, K.; Schneider, G.; Lindqvist, Y.
    Crystal structure of beta-ketoacyl-acyl carrier protein synthase II from E.coli reveals the molecular architecture of condensing enzymes (1998), EMBO J., 17, 1183-1191.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
determined with the multiple isomorphous replacement method and refined at 2.4 A resolution. Hanging drop vapor diffusion method at room temperature, using 27% PEG 8000 as precipitant, buffered at pH 7.5 with 0.1 M HEPES. The crystals grow to a size of 0.5 * 0.3 * 0.2 mm3 within 3 days. The lifetime of these crystals is very limited, they will dissolve within 10 days of their appearance. Addition of 0.1% mercaptoethanol to the reservoir solution significantly increases the life time of the crystals. Space group: P3(1)21 with cell dimensions a = 76.4 A, c = 146.8 A, gamma = 120° Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method at room temperature, using 27% PEG 8000 as precipitant and buffered at pH 7.5 with 0.1 M HEPES. Crystal structure is determined with the multiple isomorphous replacement method and refined at 2.4 A resolution, space group P3(1)21 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AAI5
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-

Subunits

Subunits Comment Organism
dimer
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Escherichia coli

Synonyms

Synonyms Comment Organism
beta-ketoacyl-acyl carrier protein synthase II
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Escherichia coli