KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, overview | Carboxydothermus hydrogenoformans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co3+ | part of the methylcorrinoid protein | Carboxydothermus hydrogenoformans | |
additional information | Ti3+ NTAis utilized in the CO exchange assay | Carboxydothermus hydrogenoformans | |
Ni2+ | required as reductant of the methylcorrinoid protein | Carboxydothermus hydrogenoformans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + corrinoid protein | Carboxydothermus hydrogenoformans | - |
CoA + CO + methylcorrinoid protein | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carboxydothermus hydrogenoformans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + corrinoid protein | - |
Carboxydothermus hydrogenoformans | CoA + CO + methylcorrinoid protein | - |
r | |
additional information | in assays of bacterial ACS, methylated corrinoid iron-sulfur protein is generally used as the source of methyl groups for acetyl-CoA synthesis | Carboxydothermus hydrogenoformans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACSCh | - |
Carboxydothermus hydrogenoformans |
CODH/ACS | - |
Carboxydothermus hydrogenoformans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
acetyl-CoA synthesis and CO exchange assay at | Carboxydothermus hydrogenoformans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
CO exchange assay at | Carboxydothermus hydrogenoformans |
7.2 | - |
acetyl-CoA synthesis assay at | Carboxydothermus hydrogenoformans |
General Information | Comment | Organism |
---|---|---|
physiological function | CODH/ACS is the only protein required to catalyze exchange of the carbonyl group of acetyl-CoA with free CO. CODH/ACS acts on both the acetyl C-C and C-S bonds in acetyl-CoA, and it identified CODH/ACS as the condensing enzyme that catalyzes the final steps in acetyl-CoA synthesis in acetogenic bacteria | Carboxydothermus hydrogenoformans |