Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | neither ATP nor phosphate stimulate the exchange | Moorella thermoacetica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'dithiobis-(2-nitrobenzoic acid) | inhibits the acetyl-CoA/CO exchange reaction | Moorella thermoacetica | |
Mersalyl acid | inhibits the acetyl-CoA/CO exchange reaction | Moorella thermoacetica | |
methyl iodide | inhibits the acetyl-CoA/CO exchange reaction | Moorella thermoacetica | |
additional information | no inhibition of the exchange reaction by methyl- and phenylglyoxal, and butanedione | Moorella thermoacetica | |
Sodium dithionite | inhibits the acetyl-CoA/CO exchange reaction | Moorella thermoacetica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Moorella thermoacetica | the purified carbon monoxide dehydrogenase, EC 1.2.7.4, from Clostridium thermoaceticum is the only protein required to catalyze a reversible exchange reaction between carbon monoxide and the carbonyl group of acetyl-CoA. Carbon dioxide also exchanges with the C-1 of acetyl-coA, but at a much lower rate than does CO | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moorella thermoacetica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by anion exchange and hydrophobic interaction chromatography, followed by hydroxylapatite chromatography, gel filtration, and CoA affinity chromatography | Moorella thermoacetica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
70 mol of CO exchanged per min/mol of enzyme | Moorella thermoacetica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the purified carbon monoxide dehydrogenase, EC 1.2.7.4, from Clostridium thermoaceticum is the only protein required to catalyze a reversible exchange reaction between carbon monoxide and the carbonyl group of acetyl-CoA. Carbon dioxide also exchanges with the C-1 of acetyl-coA, but at a much lower rate than does CO | Moorella thermoacetica | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Moorella thermoacetica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.3 | - |
- |
Moorella thermoacetica |