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Literature summary for 2.3.1.169 extracted from

  • Ragsdale, S.W.; Wood, H.G.
    Acetate biosynthesis by acetogenic bacteria. Evidence that carbon monoxide dehydrogenase is the condensing enzyme that catalyzes the final steps of the synthesis (1985), J. Biol. Chem., 260, 3970-3977.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information neither ATP nor phosphate stimulate the exchange Moorella thermoacetica

Inhibitors

Inhibitors Comment Organism Structure
5,5'dithiobis-(2-nitrobenzoic acid) inhibits the acetyl-CoA/CO exchange reaction Moorella thermoacetica
Mersalyl acid inhibits the acetyl-CoA/CO exchange reaction Moorella thermoacetica
methyl iodide inhibits the acetyl-CoA/CO exchange reaction Moorella thermoacetica
additional information no inhibition of the exchange reaction by methyl- and phenylglyoxal, and butanedione Moorella thermoacetica
Sodium dithionite inhibits the acetyl-CoA/CO exchange reaction Moorella thermoacetica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Moorella thermoacetica the purified carbon monoxide dehydrogenase, EC 1.2.7.4, from Clostridium thermoaceticum is the only protein required to catalyze a reversible exchange reaction between carbon monoxide and the carbonyl group of acetyl-CoA. Carbon dioxide also exchanges with the C-1 of acetyl-coA, but at a much lower rate than does CO ?
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?

Organism

Organism UniProt Comment Textmining
Moorella thermoacetica
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Purification (Commentary)

Purification (Comment) Organism
native enzyme by anion exchange and hydrophobic interaction chromatography, followed by hydroxylapatite chromatography, gel filtration, and CoA affinity chromatography Moorella thermoacetica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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70 mol of CO exchanged per min/mol of enzyme Moorella thermoacetica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the purified carbon monoxide dehydrogenase, EC 1.2.7.4, from Clostridium thermoaceticum is the only protein required to catalyze a reversible exchange reaction between carbon monoxide and the carbonyl group of acetyl-CoA. Carbon dioxide also exchanges with the C-1 of acetyl-coA, but at a much lower rate than does CO Moorella thermoacetica ?
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?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
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Moorella thermoacetica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3
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-
Moorella thermoacetica