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Literature summary for 2.3.1.167 extracted from

  • Loncaric, C.; Ward, A.F.; Walker, K.D.
    Expression of an acetyl-CoA synthase and a CoA-transferase in Escherichia coli to produce modified taxanes in vivo (2007), Biotechnol. J., 2, 266-274.
    View publication on PubMed
Search for more literature for 2.3.1.167

Application

Application Comment Organism
biotechnology optimizing the semi-biosynthetic method in bacteria potentially provides a practical means of developing commercial-scale production of baccatin III analogs, which serve as key intermediates in the semi-synthesis of second-generation taxols Taxus sp.
synthesis bioengineered Escherichia coli cells synthesize nonnatural 10-deacetyl-10-acylbaccatin III derivatives as potential precursors of second-generation Taxol derivatives using the enzyme in concert with acyl-CoA transferases, determination of optimal alkanoic acid concentration for in vivo production of baccatin III analogues, overview Taxus sp.

Cloned(Commentary)

Cloned (Comment) Organism
gene dbat, functional expression in Escherichia coli strain JM109, co-expression with acetyl-CoA synthase, gene acs, EC 6.2.1.1, or with acetoacetate:acetyl-CoA CoA-transferase, genes atoAD of the ato operon, EC 2.8.3.8, expressed from and regulated by genes encoded on the bacterial chromosome, overview Taxus sp.
into the vector pCWori+ for expression in Escherichia coli JM109 and BL21DE3 cells Taxus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + 10-deacetylbaccatin III Taxus sp.
-
 CoA + baccatin III
-
 ?
butyryl-CoA + 10-deacetylbaccatin III Taxus sp.
-
 CoA + 10-deacetyl-10-butyrylbaccatin III
-
 ?
propionyl-CoA + 10-deacetylbaccatin III Taxus sp.
-
 CoA + 10-deacetyl-10-propionylbaccatin III
-
 ?

Organism

Organism UniProt Comment Textmining
Taxus sp.
-
-
-
Taxus sp.
-
 Taxus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 10-deacetylbaccatin III
-
 Taxus sp. CoA + baccatin III
-
 ?
butyryl-CoA + 10-deacetylbaccatin III
-
 Taxus sp. CoA + 10-deacetyl-10-butyrylbaccatin III
-
 ?
additional information DBAT catalyzes the transfer of acetyl, propionyl or n-butyryl from CoA to the C10-hydroxyl of 10-deacetylbaccatin III Taxus sp. ?
-
 ?
n-butyryl-CoA + 10-deacetylbaccatin III
-
 Taxus sp. CoA + 10-deacetyl-10-butyrylbaccatin III
-
 ?
propionyl-CoA + 10-deacetylbaccatin III
-
 Taxus sp. CoA + 10-deacetyl-10-propionylbaccatin III
-
 ?

Synonyms

Synonyms Comment Organism
10-deacetylbaccatin III 10-O-acetyltransferase
-
 Taxus sp.
10-deacetylbaccatin III 10beta-O-acetyltransferase
-
 Taxus sp.
DBAT
-
 Taxus sp.

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Taxus sp.