Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Penicillium chrysogenum |
Crystallization (Comment) | Organism |
---|---|
- |
Penicillium chrysogenum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
11000 | - |
1 * 11000 + 1 * 29000, SDS-PAGE | Penicillium chrysogenum |
29000 | - |
1 * 11000 + 1 * 29000, SDS-PAGE | Penicillium chrysogenum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium chrysogenum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type enzyme with a C-terminal His-tag is purified in a single step using Ni2+ affinity chelate chromatography. A combination of 5 mM DTT + 250 mM NaCl + 5 mM EDTA is effective in preventing aggregation of the enzyme | Penicillium chrysogenum |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 11000 + 1 * 29000, SDS-PAGE | Penicillium chrysogenum |
Synonyms | Comment | Organism |
---|---|---|
acyl coenzyme A: isopenicillin N acyltransferase | - |
Penicillium chrysogenum |