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Literature summary for 2.3.1.16 extracted from

  • Teufel, R.; Mascaraque, V.; Ismail, W.; Voss, M.; Perera, J.; Eisenreich, W.; Haehnel, W.; Fuchs, G.
    Bacterial phenylalanine and phenylacetate catabolic pathway revealed (2010), Proc. Natl. Acad. Sci. USA, 107, 14390-14395.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.16

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of tagged enzyme Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 gene paaJ
-
Pseudomonas sp. Y2
-
 gene paaJ
-

Purification (Commentary)

Purification (Comment) Organism
recombinant tagged enzyme by affinity chromatography Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-oxo-5,6-dehydrosuberyl-CoA + CoA
-
 Pseudomonas sp. 2,3-dehydroadipyl-CoA + acetyl-CoA
-
 ?
3-oxo-5,6-dehydrosuberyl-CoA + CoA
-
 Pseudomonas sp. Y2 2,3-dehydroadipyl-CoA + acetyl-CoA
-
 ?

Synonyms

Synonyms Comment Organism
3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase
-
 Pseudomonas sp.
beta-ketothiolase
-
 Pseudomonas sp.
PaaJ
-
 Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Pseudomonas sp.

General Information

General Information Comment Organism
metabolism the enzyme PaaJ is involved in the aerobic phenylacetate catabolic pathway. PaaJ, a beta-ketothiolase, transforms the resulting beta-keto C8 intermediate with CoA to the C6 intermediate dehydroadipyl-CoA and acetyl-CoA, besides catalyzing the last step of the pathway, in which 3-oxoadipyl-CoA similarly is cleaved to acetyl-CoA and succinyl-CoA Pseudomonas sp.