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Literature summary for 2.3.1.16 extracted from

  • Schulz, H.; Staack, H.
    3-Ketoacyl-Co-A-thiolase with broad chain length specificity from pig heart muscle (1981), Methods Enzymol., 71, 389-403.
No PubMed abstract available
Search for more literature for 2.3.1.16

General Stability

General Stability Organism
glycerol or 2-mercaptoethanol stabilizes Sus scrofa
inactivation at Tris-HCl concentration Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide
-
 Sus scrofa
long-chain 3-oxoacyl-CoA compounds
-
 Sus scrofa
N-Methylmaleimide
-
 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
 3-oxodecanoyl-CoA
-
 Sus scrofa
0.0024
-
 3-oxooctanoyl-CoA
-
 Sus scrofa
0.0083
-
 3-oxohexanoyl-CoA
-
 Sus scrofa
0.0087
-
 CoA-SH with acetoacetyl-CoA Sus scrofa
0.017
-
 acetoacetyl-CoA
-
 Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Sus scrofa 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 4 * 46000, SDS-PAGE Sus scrofa
200000
-
 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + 3-oxoacyl-CoA Sus scrofa enzyme catalyses the last step in the beta-oxidation cycle acyl-CoA + acetyl-CoA
-
 r

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 assay method Sus scrofa
597
-
-
 Sus scrofa

Storage Stability

Storage Stability Organism
25°C, 0.75 M Tris-HCl, pH 8.1, 25% v/v glycerol, 10 mM 2-mercaptoethanol, stable for days Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + 3-oxoacyl-CoA
-
 Sus scrofa acyl-CoA + acetyl-CoA
-
 r
CoA + 3-oxoacyl-CoA enzyme catalyses the last step in the beta-oxidation cycle Sus scrofa acyl-CoA + acetyl-CoA
-
 r
CoA + 3-oxodecanoyl-CoA
-
 Sus scrofa acetyl-CoA + octanoyl-CoA
-
 r
CoA + 3-oxohexanoyl-CoA
-
 Sus scrofa acetyl-CoA + butanoyl-CoA
-
 r
CoA + 3-oxooctanoyl-CoA
-
 Sus scrofa acetyl-CoA + hexanoyl-CoA
-
 r
CoA + acetoacetyl-CoA
-
 Sus scrofa acetyl-CoA + acetyl-CoA
-
 r
additional information acetoacetyl-CoA is a poor substrate Sus scrofa ?
-
 ?
additional information acts on 3-oxoacyl-CoA compounds of various chain lengths Sus scrofa ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 46000, SDS-PAGE Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 pH 8.1, 25% glycerol v/v, stable for days Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
7.6 8.3 pH 7.6: maximum activity, pH 8.3: about 75% of maximum activity Sus scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.1
-
 25°C, 25% glycerol v/v, stable for days Sus scrofa