Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Polymyxin B | - |
Macrobrachium borellii |
General Stability | Organism |
---|---|
since enzyme is unstable after long freezing periods, all experiments are performed within two weeks of fractionation | Macrobrachium borellii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
palmitoleoyl-CoA | shows substrate inhibition, therfore Km value is not determined | Macrobrachium borellii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0312 | - |
oleoyl-CoA | Vmax value calculated with oleoyl-CoA as a substrate is 4fold lower than the value obtained with palmitoyl-CoA | Macrobrachium borellii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | higly active in mitochondria | Macrobrachium borellii | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
SDS-PAGE | Macrobrachium borellii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Macrobrachium borellii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
enzyme is isolated using subcellular fractions | Macrobrachium borellii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hepatopancreas | in hepatopancreas mitochondria, a GPAT activity with characteristics similar to those of mammalian GPAT1 is identified | Macrobrachium borellii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000023 | - |
specific activity in total homogenate | Macrobrachium borellii |
0.0000342 | - |
specific activity in microsomes | Macrobrachium borellii |
0.000322 | - |
specific activity in mitochondria | Macrobrachium borellii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oleoyl-CoA + sn-glycerol 3-phosphate | - |
Macrobrachium borellii | CoA + 1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | preferred substrate is palmitoyl-CoA | Macrobrachium borellii | CoA + 1-palmitoleoyl-sn-glycerol 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glycerol-3-phosphate acyltransferase | - |
Macrobrachium borellii |
GPAT | - |
Macrobrachium borellii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Macrobrachium borellii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Macrobrachium borellii |