Inhibitors | Comment | Organism | Structure |
---|---|---|---|
palmitoyl-CoA | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
palmitoyl-CoA | value below 0.007 mM, fixed substrate concentration: 0.002 mM glycerol-3-phosphate, without addition of insulin, Vmax: 2.4 nmol/min/mg | Rattus norvegicus | |
0.0264 | - |
palmitoyl-CoA | fixed substrate concentration: 0.002 mM glycerol-3-phosphate, with addition of insulin, Vmax: 14.1 nmol/min/mg | Rattus norvegicus | |
3.9 | - |
glycerol 3-phosphate | fixed substrate concentration: 0.08 mM palmitoyl-CoA, without addition of insulin, Vmax: 6.1 nmol/min/mg | Rattus norvegicus | |
7.2 | - |
glycerol 3-phosphate | fixed substrate concentration: 0.02 mM palmitoyl-CoA, with addition of insulin, Vmax: 7.2 nmol/min/mg | Rattus norvegicus | |
16.4 | - |
glycerol 3-phosphate | fixed substrate concentration: 0.08 mM palmitoyl-CoA, with addition of insulin, Vmax: 16.6 nmol/min/mg | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | proteolytic cleavage of isolated mitochondrial membranes and mass spectrometric peptide sequencing identify in vivo phosphorylation of serine 632 and serine 639 in mtGPAT1. These phosphorylation sites correspond to casein kinase-2 consensus sequences | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adipocyte | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + glycerol 3-phosphate | - |
Rattus norvegicus | CoA + 1-palmitoyl-glycerol 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mtGPAT1 | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay | Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0945 | - |
with addition of insulin | Rattus norvegicus | palmitoyl-CoA | |
0.126 | - |
without addition of insulin | Rattus norvegicus | palmitoyl-CoA |
General Information | Comment | Organism |
---|---|---|
physiological function | treatment of primary rat adipocytes with insulin acutely affects the activity of mtGPAT1 by increasing VMAX and KM for the substrates glycerol-3-phosphate and palmitoyl-CoA | Rattus norvegicus |