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Literature summary for 2.3.1.15 extracted from

  • Zufferey, R.; Mamoun, C.B.
    The initial step of glycerolipid metabolism in Leishmania major promastigotes involves a single glycerol-3-phosphate acyltransferase enzyme important for the synthesis of triacylglycerol but not essential for virulence (2005), Mol. Microbiol., 56, 800-810.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, full functional complementation by expression of His6-tagged enzyme in an enzyme-deficient yeast mutant strain BYL118, the recombinant enzyme shows a slightly altered substrate specificity, overview Leishmania major

Protein Variants

Protein Variants Comment Organism
additional information construction of an enzyme deletion mutant, which is inactive in enzyme activity and highly reduced in triacylglycerol synthesis, but not in other lipid or virulence factor synthesis, overview Leishmania major

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics, cell extract Leishmania major
0.69
-
sn-glycerol 3-phosphate pH 7.5, 26°C, recombinant enzyme in cell extracts of enzyme-deficient yeast mutant cells strain BYL118 Leishmania major
2.08
-
sn-glycerol 3-phosphate pH 7.5, 26°C, wild-type enzyme in cell extracts of promastigote Leishmania major

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Leishmania major 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
DTT
-
Leishmania major
Mg2+
-
Leishmania major

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
x * 75000, recombinant enzyme, SDS-PAGE Leishmania major

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Leishmania major enzyme important for the synthesis of triacylglycerol but not essential for virulence ?
-
?
palmitoyl-CoA + sn-glycerol 3-phosphate Leishmania major
-
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Leishmania major
-
single gene, no isozymes
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information high enzyme activity during the exponential growth phase of the parasite, but low during stationary phase Leishmania major
-
promastigote
-
Leishmania major
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidoyl-CoA + sn-glycerol 3-phosphate
-
Leishmania major CoA + 1-arachidoyl-sn-glycerol 3-phosphate
-
?
lauroyl-CoA + sn-glycerol 3-phosphate
-
Leishmania major CoA + 1-lauroyl-sn-glycerol 3-phosphate
-
?
linoleoyl-CoA + sn-glycerol 3-phosphate high activity Leishmania major CoA + 1-linoleoyl-sn-glycerol 3-phosphate
-
?
additional information enzyme important for the synthesis of triacylglycerol but not essential for virulence Leishmania major ?
-
?
myristoyl-CoA + sn-glycerol 3-phosphate
-
Leishmania major CoA + 1-myristoyl-sn-glycerol 3-phosphate
-
?
oleoyl-CoA + sn-glycerol 3-phosphate high activity Leishmania major CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
?
palmitoleoyl-CoA + sn-glycerol 3-phosphate high activity Leishmania major CoA + 1-palmitoleoyl-sn-glycerol 3-phosphate
-
?
palmitoyl-CoA + sn-glycerol 3-phosphate
-
Leishmania major CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
?
palmitoyl-CoA + sn-glycerol 3-phosphate high activity Leishmania major CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
?
stearoyl-CoA + sn-glycerol 3-phosphate high activity Leishmania major CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 75000, recombinant enzyme, SDS-PAGE Leishmania major

Synonyms

Synonyms Comment Organism
GAT
-
Leishmania major
glycerol-3-phosphate acyltransferase
-
Leishmania major

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
26
-
assay at Leishmania major

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Leishmania major