Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, full functional complementation by expression of His6-tagged enzyme in an enzyme-deficient yeast mutant strain BYL118, the recombinant enzyme shows a slightly altered substrate specificity, overview | Leishmania major |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an enzyme deletion mutant, which is inactive in enzyme activity and highly reduced in triacylglycerol synthesis, but not in other lipid or virulence factor synthesis, overview | Leishmania major |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, cell extract | Leishmania major | |
0.69 | - |
sn-glycerol 3-phosphate | pH 7.5, 26°C, recombinant enzyme in cell extracts of enzyme-deficient yeast mutant cells strain BYL118 | Leishmania major | |
2.08 | - |
sn-glycerol 3-phosphate | pH 7.5, 26°C, wild-type enzyme in cell extracts of promastigote | Leishmania major |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Leishmania major | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
DTT | - |
Leishmania major | |
Mg2+ | - |
Leishmania major |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
x * 75000, recombinant enzyme, SDS-PAGE | Leishmania major |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Leishmania major | enzyme important for the synthesis of triacylglycerol but not essential for virulence | ? | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | Leishmania major | - |
CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | - |
single gene, no isozymes | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | high enzyme activity during the exponential growth phase of the parasite, but low during stationary phase | Leishmania major | - |
promastigote | - |
Leishmania major | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidoyl-CoA + sn-glycerol 3-phosphate | - |
Leishmania major | CoA + 1-arachidoyl-sn-glycerol 3-phosphate | - |
? | |
lauroyl-CoA + sn-glycerol 3-phosphate | - |
Leishmania major | CoA + 1-lauroyl-sn-glycerol 3-phosphate | - |
? | |
linoleoyl-CoA + sn-glycerol 3-phosphate | high activity | Leishmania major | CoA + 1-linoleoyl-sn-glycerol 3-phosphate | - |
? | |
additional information | enzyme important for the synthesis of triacylglycerol but not essential for virulence | Leishmania major | ? | - |
? | |
myristoyl-CoA + sn-glycerol 3-phosphate | - |
Leishmania major | CoA + 1-myristoyl-sn-glycerol 3-phosphate | - |
? | |
oleoyl-CoA + sn-glycerol 3-phosphate | high activity | Leishmania major | CoA + 1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoleoyl-CoA + sn-glycerol 3-phosphate | high activity | Leishmania major | CoA + 1-palmitoleoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | - |
Leishmania major | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | high activity | Leishmania major | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
stearoyl-CoA + sn-glycerol 3-phosphate | high activity | Leishmania major | CoA + 1-stearoyl-sn-glycerol 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 75000, recombinant enzyme, SDS-PAGE | Leishmania major |
Synonyms | Comment | Organism |
---|---|---|
GAT | - |
Leishmania major |
glycerol-3-phosphate acyltransferase | - |
Leishmania major |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
26 | - |
assay at | Leishmania major |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Leishmania major |