Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Polymyxin B | antibiotic, reversible binding, stimulation via enhanced extraction of lysophosphatidic acid, maximal at 60%, the acyl side group does not play a role in stimulation, addition of bovine serum albumin is highly required for stimulation, in absence of bovine serum albumin the antibiotic is slightly inhibitory, addition of Mg2+ enhances the stimulation | Rattus norvegicus | |
spermidine | activates about 3.5fold in presence of 0.1 mg/ml bovine serum albumin | Rattus norvegicus | |
spermine | activates about 3fold in presence of 0.1 mg/ml bovine serum albumin | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Polymyxin B | in absence of BSA the antibiotic is slightly inhibitory | Rattus norvegicus | |
spermidine | inhibits slightly in absence of bovine serum albumin | Rattus norvegicus | |
spermine | inhibits slightly in absence of bovine serum albumin | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Rattus norvegicus | 5783 | - |
mitochondrial outer membrane | - |
Rattus norvegicus | 5741 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates in presence of polymyxin | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | Rattus norvegicus | the enzyme catalyzes the first and committed step in the synthesis of glycerophospholipids and triacylglycerol from sn-glycerol-3-phosphate | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male Harlan Sprague-Dawley rats | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
0.0027 | - |
about, enzyme in presence of 10 mM MgCl2 and 0.1 mg/ml polymyxin | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | the enzyme catalyzes the first and committed step in the synthesis of glycerophospholipids and triacylglycerol from sn-glycerol-3-phosphate | Rattus norvegicus | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | - |
Rattus norvegicus | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | i.e. lysophosphatidic acid | ? |
Synonyms | Comment | Organism |
---|---|---|
GAT | - |
Rattus norvegicus |
glycerophosphate acyltransferase | - |
Rattus norvegicus |
sn-glycerol-3-phosphate acyltransferase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |