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Literature summary for 2.3.1.15 extracted from

  • Roy, A.; Guha, N.; Veras, I.D.; Chakraborty, S.; Haldar, D.
    Stimulation of rat liver mitochondrial sn-glycerol-3-phosphate acyltransferase by polymyxin B via enhanced extraction of lysophosphatidic acid (2003), Lipids, 38, 965-972.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Polymyxin B antibiotic, reversible binding, stimulation via enhanced extraction of lysophosphatidic acid, maximal at 60%, the acyl side group does not play a role in stimulation, addition of bovine serum albumin is highly required for stimulation, in absence of bovine serum albumin the antibiotic is slightly inhibitory, addition of Mg2+ enhances the stimulation Rattus norvegicus
spermidine activates about 3.5fold in presence of 0.1 mg/ml bovine serum albumin Rattus norvegicus
spermine activates about 3fold in presence of 0.1 mg/ml bovine serum albumin Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Polymyxin B in absence of BSA the antibiotic is slightly inhibitory Rattus norvegicus
spermidine inhibits slightly in absence of bovine serum albumin Rattus norvegicus
spermine inhibits slightly in absence of bovine serum albumin Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
Rattus norvegicus 5783
-
mitochondrial outer membrane
-
Rattus norvegicus 5741
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates in presence of polymyxin Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + sn-glycerol 3-phosphate Rattus norvegicus the enzyme catalyzes the first and committed step in the synthesis of glycerophospholipids and triacylglycerol from sn-glycerol-3-phosphate CoA + 1-acyl-sn-glycerol 3-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
male Harlan Sprague-Dawley rats
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Rattus norvegicus
0.0027
-
about, enzyme in presence of 10 mM MgCl2 and 0.1 mg/ml polymyxin Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + sn-glycerol 3-phosphate the enzyme catalyzes the first and committed step in the synthesis of glycerophospholipids and triacylglycerol from sn-glycerol-3-phosphate Rattus norvegicus CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
palmitoyl-CoA + sn-glycerol 3-phosphate
-
Rattus norvegicus CoA + 1-palmitoyl-sn-glycerol 3-phosphate i.e. lysophosphatidic acid ?

Synonyms

Synonyms Comment Organism
GAT
-
Rattus norvegicus
glycerophosphate acyltransferase
-
Rattus norvegicus
sn-glycerol-3-phosphate acyltransferase
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Rattus norvegicus