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Literature summary for 2.3.1.15 extracted from

  • Tamada, T.; Feese, M.D.; Ferri, S.R.; Kato, Y.; Yajima, R.; Toguri, T.; Kuroki, R.
    Substrate recognition and selectivity of plant glycerol-3-phosphate acyltransferases (GPATs) from Cucurbita moschata and Spinacia oleracea (2004), Acta Crystallogr. Sect. D, 60, 13-21.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of chimeric mature enzyme containing residues 128-187 from Spinacia oleracea and residues 1-127 from Cucurbita moscata, in Escherichia coli strain BL21(DE3) Spinacia oleracea
expression of native and selenomethionine-labeled wild-type and chimeric mature enzymes, the latter contains residues 128-187 from Spinacia oleracea and residues 1-127 from Cucurbita moscata, in Escherichia coli strain BL21(DE3) and a nonauxotrophic strain Cucurbita moschata

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant native and selenomethionine-labeled wild-type and chimeric enzymes, 14 mg/ml protein in 20 mM sodium phosphate, pH 8.0, 4°C, hanging drop vapour diffusion method, crystallization solution contains 4-25% PEG 4000, 0.1 M ammonium acetate,10% v/v 2-propanolin 100 mM citrate, pH 5.6, X-ray diffraction structure determination and analysis at 1.55 A resolution Cucurbita moschata
recombinant chimeric enzyme, 14 mg/ml protein in 20 mM sodium phosphate, pH 8.0, 4°C, hanging drop vapour diffusion method, crystallization solution contains 4-25% PEG 4000, 0.1 M ammonium acetate,10% v/v 2-propanolin 100 mM citrate, pH 5.6, X-ray diffraction structure determination and analysis at 1.55 A resolution Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast stroma Spinacia oleracea 9507
-
chloroplast stroma Cucurbita moschata 9507
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + sn-glycerol 3-phosphate Spinacia oleracea first committed step in biosynthesis of fatty acid biosynthesis, the enzyme is responsible for incorporation of saturated and unsaturated fatty-acyl chains into chloroplast membranes CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
acyl-CoA + sn-glycerol 3-phosphate Cucurbita moschata first committed step in biosynthesis of fatty acid biosynthesis, the enzyme is responsible for incorporation of saturated and unsaturated fatty-acyl chains into chloroplast membranes CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
additional information Spinacia oleracea the content of cis-unsaturated fatty acids in the plastidial phosphatidyl glycerol determines the degree of sensitivity to chilling, the higher it is the more tolerant are the plants to chilling, spinach is chilling-insensitive, while squash is chilling-sensitive, overview ?
-
?
additional information Cucurbita moschata the content of cis-unsaturated fatty acids in the plastidial phosphatidyl glycerol determines the degree of sensitivity to chilling, the higher it is the more tolerant are the plants to chilling, squash is chilling-sensitive, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Cucurbita moschata
-
-
-
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant chimeric mature enzyme Spinacia oleracea
recombinant native and selenomethionine-labeled wild-type and chimeric mature enzymes by anio exchange chromatography Cucurbita moschata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + sn-glycerol 3-phosphate
-
Spinacia oleracea CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
acyl-CoA + sn-glycerol 3-phosphate
-
Cucurbita moschata CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
acyl-CoA + sn-glycerol 3-phosphate first committed step in biosynthesis of fatty acid biosynthesis, the enzyme is responsible for incorporation of saturated and unsaturated fatty-acyl chains into chloroplast membranes Spinacia oleracea CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
acyl-CoA + sn-glycerol 3-phosphate first committed step in biosynthesis of fatty acid biosynthesis, the enzyme is responsible for incorporation of saturated and unsaturated fatty-acyl chains into chloroplast membranes Cucurbita moschata CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
additional information the content of cis-unsaturated fatty acids in the plastidial phosphatidyl glycerol determines the degree of sensitivity to chilling, the higher it is the more tolerant are the plants to chilling, spinach is chilling-insensitive, while squash is chilling-sensitive, overview Spinacia oleracea ?
-
?
additional information the content of cis-unsaturated fatty acids in the plastidial phosphatidyl glycerol determines the degree of sensitivity to chilling, the higher it is the more tolerant are the plants to chilling, squash is chilling-sensitive, overview Cucurbita moschata ?
-
?
additional information substrate specificity of the chimeric mutant enzymes, overview Spinacia oleracea ?
-
?
additional information substrate specificity of wild-type and chimeric mutant enzymes, overview Cucurbita moschata ?
-
?

Synonyms

Synonyms Comment Organism
glycerol-3-phosphate acyltransferase
-
Spinacia oleracea
glycerol-3-phosphate acyltransferase
-
Cucurbita moschata
GPAT
-
Spinacia oleracea
GPAT
-
Cucurbita moschata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Spinacia oleracea
25
-
assay at Cucurbita moschata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Spinacia oleracea
7.4
-
assay at Cucurbita moschata