Cloned (Comment) | Organism |
---|---|
expression of chimeric mature enzyme containing residues 128-187 from Spinacia oleracea and residues 1-127 from Cucurbita moscata, in Escherichia coli strain BL21(DE3) | Spinacia oleracea |
expression of native and selenomethionine-labeled wild-type and chimeric mature enzymes, the latter contains residues 128-187 from Spinacia oleracea and residues 1-127 from Cucurbita moscata, in Escherichia coli strain BL21(DE3) and a nonauxotrophic strain | Cucurbita moschata |
Crystallization (Comment) | Organism |
---|---|
purified recombinant native and selenomethionine-labeled wild-type and chimeric enzymes, 14 mg/ml protein in 20 mM sodium phosphate, pH 8.0, 4°C, hanging drop vapour diffusion method, crystallization solution contains 4-25% PEG 4000, 0.1 M ammonium acetate,10% v/v 2-propanolin 100 mM citrate, pH 5.6, X-ray diffraction structure determination and analysis at 1.55 A resolution | Cucurbita moschata |
recombinant chimeric enzyme, 14 mg/ml protein in 20 mM sodium phosphate, pH 8.0, 4°C, hanging drop vapour diffusion method, crystallization solution contains 4-25% PEG 4000, 0.1 M ammonium acetate,10% v/v 2-propanolin 100 mM citrate, pH 5.6, X-ray diffraction structure determination and analysis at 1.55 A resolution | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | stroma | Spinacia oleracea | 9507 | - |
chloroplast | stroma | Cucurbita moschata | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | Spinacia oleracea | first committed step in biosynthesis of fatty acid biosynthesis, the enzyme is responsible for incorporation of saturated and unsaturated fatty-acyl chains into chloroplast membranes | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | Cucurbita moschata | first committed step in biosynthesis of fatty acid biosynthesis, the enzyme is responsible for incorporation of saturated and unsaturated fatty-acyl chains into chloroplast membranes | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
additional information | Spinacia oleracea | the content of cis-unsaturated fatty acids in the plastidial phosphatidyl glycerol determines the degree of sensitivity to chilling, the higher it is the more tolerant are the plants to chilling, spinach is chilling-insensitive, while squash is chilling-sensitive, overview | ? | - |
? | |
additional information | Cucurbita moschata | the content of cis-unsaturated fatty acids in the plastidial phosphatidyl glycerol determines the degree of sensitivity to chilling, the higher it is the more tolerant are the plants to chilling, squash is chilling-sensitive, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cucurbita moschata | - |
- |
- |
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant chimeric mature enzyme | Spinacia oleracea |
recombinant native and selenomethionine-labeled wild-type and chimeric mature enzymes by anio exchange chromatography | Cucurbita moschata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | - |
Spinacia oleracea | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | - |
Cucurbita moschata | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | first committed step in biosynthesis of fatty acid biosynthesis, the enzyme is responsible for incorporation of saturated and unsaturated fatty-acyl chains into chloroplast membranes | Spinacia oleracea | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | first committed step in biosynthesis of fatty acid biosynthesis, the enzyme is responsible for incorporation of saturated and unsaturated fatty-acyl chains into chloroplast membranes | Cucurbita moschata | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
additional information | the content of cis-unsaturated fatty acids in the plastidial phosphatidyl glycerol determines the degree of sensitivity to chilling, the higher it is the more tolerant are the plants to chilling, spinach is chilling-insensitive, while squash is chilling-sensitive, overview | Spinacia oleracea | ? | - |
? | |
additional information | the content of cis-unsaturated fatty acids in the plastidial phosphatidyl glycerol determines the degree of sensitivity to chilling, the higher it is the more tolerant are the plants to chilling, squash is chilling-sensitive, overview | Cucurbita moschata | ? | - |
? | |
additional information | substrate specificity of the chimeric mutant enzymes, overview | Spinacia oleracea | ? | - |
? | |
additional information | substrate specificity of wild-type and chimeric mutant enzymes, overview | Cucurbita moschata | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glycerol-3-phosphate acyltransferase | - |
Spinacia oleracea |
glycerol-3-phosphate acyltransferase | - |
Cucurbita moschata |
GPAT | - |
Spinacia oleracea |
GPAT | - |
Cucurbita moschata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Spinacia oleracea |
25 | - |
assay at | Cucurbita moschata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Spinacia oleracea |
7.4 | - |
assay at | Cucurbita moschata |