Cloned (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
- |
Cucurbita moschata |
- |
Elaeis guineensis |
Protein Variants | Comment | Organism |
---|---|---|
E142A | no activity | Cucurbita moschata |
H194S | 79% of wild-type activity | Cucurbita moschata |
K193S | no activity | Cucurbita moschata |
L261F | 90% of wild-type activity | Cucurbita moschata |
L261F | change from a non-selective acyltransferase to an oleate-selective acyltransferase | Cucurbita moschata |
L352F | change from a non-selective acyltransferase to an oleate-selective acyltransferase, 117% of wild-type activity | Elaeis guineensis |
R235S | no activity | Cucurbita moschata |
R237S | no activity | Cucurbita moschata |
S331P | no change in acyl-donor selectivity | Cucurbita moschata |
S331P | 94% of wild-type activity | Cucurbita moschata |
T141S | 106% of wild-type activity | Cucurbita moschata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
oleoyl-ACP | L261F mutant acyltransferase | Cucurbita moschata | |
0.003 | - |
oleoyl-ACP | - |
Cucurbita moschata | |
0.0034 | - |
palmitoyl-[acyl carrier protein] | - |
Cucurbita moschata | |
0.0092 | - |
palmitoyl-[acyl carrier protein] | L261F mutant acyltransferase | Cucurbita moschata | |
0.11 | - |
glycerol 3-phosphate | L261F mutant acyltransferase, acyl-donor oleoyl-CoA | Cucurbita moschata | |
0.117 | 0.143 | glycerol 3-phosphate | acyl donor palmitoyl-CoA | Cucurbita moschata | |
0.12 | - |
glycerol 3-phosphate | acyl donor oleoyl-CoA | Cucurbita moschata | |
0.143 | - |
glycerol 3-phosphate | acyl donor palmitoyl-CoA | Cucurbita moschata | |
0.15 | - |
glycerol 3-phosphate | L261F mutant acyltransferase, acyl-donor palmitoyl-CoA | Cucurbita moschata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Cucurbita moschata | - |
- |
- |
Elaeis guineensis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oleoyl-CoA + sn-glycerol 3-phosphate | - |
Cucurbita moschata | CoA + 1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
oleoyl-CoA + sn-glycerol 3-phosphate | preferred over palmitoyl-CoA | Arabidopsis thaliana | CoA + 1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-[acyl carrier protein] + sn-glycerol 3-phosphate | - |
Cucurbita moschata | holo-[acyl-carrier protein] + 1-palmitoylglycerol 3-phosphate | - |
? |