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Literature summary for 2.3.1.15 extracted from

  • Nishida, I.; Sugiura, M.; Enju, A.; Nakamura, M.
    A second gene for acyl-(acyl-carrier-protein): glycerol-3-phosphate acyltransferase in squash, Cucurbita moschata cv. Shirogikuza(*), codes for an oleate-selective isozyme: molecular cloning and protein purification studies (2000), Plant Cell Physiol., 41, 1381-1391.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39500
-
x * 40500 + x * 40000 + x * 39500, isoenzyme AT1, SDS-PAGE, immunoblot Cucurbita moschata
40000
-
x * 40500 + x * 40000 + x * 39500, isoenzyme AT1, SDS-PAGE, immunoblot Cucurbita moschata
40500
-
x * 40500 + x * 40000 + x * 39500, isoenzyme AT1, SDS-PAGE, immunoblot Cucurbita moschata

Organism

Organism UniProt Comment Textmining
Cucurbita moschata Q9FEP9 cv. Shirogikuza, CmATS1,2 encoding non-selective isoforms AT2 and AT3
-
Cucurbita moschata Q9FEQ0 cv. Shirogikuza, CmATS1,1 encoding oleate-selective isozyme AT1
-

Purification (Commentary)

Purification (Comment) Organism
isoenzyme AT1, DEAE-Toyopearl, [acyl-carrier protein]-affinity chromatography, MonoP Cucurbita moschata

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
Cucurbita moschata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10
-
isoenzyme AT1, approx. value Cucurbita moschata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oleoyl-CoA + sn-glycerol 3-phosphate isoenzyme AT1, preferred over palmitoyl-CoA Cucurbita moschata CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 40500 + x * 40000 + x * 39500, isoenzyme AT1, SDS-PAGE, immunoblot Cucurbita moschata