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Literature summary for 2.3.1.15 extracted from
- Purification and reconstitution of murine mitochondrial glycerol-3-phosphate acyltransferase. Functional expression in baculovirus-infected insect cells (1995), Biochemistry, 34, 7303-7310.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of mitochondrial acyl transferase in Sf9 insect cells | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | recombinant mitochondrial acyl transferase, not inhibited by N-ethylmaleimide | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.67 | - |
glycerol 3-phosphate | mitochondrial acyl transferase | Mus musculus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Mus musculus | 5739 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged acyl transferase, Ni2+-affinity column | Mus musculus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| reconstitution effectiveness of phospholipids in descending order: crude soybean phosphatidylcholin, phosphatidylserine, phosphatidylinositol, phosphatidylethanolamine, cardiolipin | Mus musculus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.192 | - |
recombinant mitochondrial acyl transferase | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + sn-glycerol 3-phosphate | no activity with hexanoyl-CoA, enzyme prefers saturated fatty acyl-CoAs | Mus musculus | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
| palmitoyl-CoA + sn-glycerol 3-phosphate | best acyl-donor | Mus musculus | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
| stearoyl-CoA + sn-glycerol 3-phosphate | less than 20% of activity with palmitoyl-CoA | Mus musculus | CoA + 1-stearoyl-sn-glycerol 3-phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | mitochondrial acyl transferase | Mus musculus |
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