Cloned (Comment) | Organism |
---|---|
expression of mitochondrial acyl transferase in Sf9 insect cells | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | recombinant mitochondrial acyl transferase, not inhibited by N-ethylmaleimide | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.67 | - |
glycerol 3-phosphate | mitochondrial acyl transferase | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Mus musculus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged acyl transferase, Ni2+-affinity column | Mus musculus |
Renatured (Comment) | Organism |
---|---|
reconstitution effectiveness of phospholipids in descending order: crude soybean phosphatidylcholin, phosphatidylserine, phosphatidylinositol, phosphatidylethanolamine, cardiolipin | Mus musculus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.192 | - |
recombinant mitochondrial acyl transferase | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | no activity with hexanoyl-CoA, enzyme prefers saturated fatty acyl-CoAs | Mus musculus | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | best acyl-donor | Mus musculus | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
stearoyl-CoA + sn-glycerol 3-phosphate | less than 20% of activity with palmitoyl-CoA | Mus musculus | CoA + 1-stearoyl-sn-glycerol 3-phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | mitochondrial acyl transferase | Mus musculus |