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Literature summary for 2.3.1.15 extracted from

  • Scheideler, M.A.; Bell, R.M.
    Glycerophosphate acyltransferase from Escherichia coli (1992), Methods Enzymol., 209, 55-63.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin alleviates detergent effect of palmitoyl-CoA Escherichia coli
bovine serum albumin not necessary with acyl-ACP derivatives or mixed micellar samples Escherichia coli
bovine serum albumin alleviates detergent effect of palmitoyl-CoA and oleoyl-CoA Escherichia coli
phosphatidylethanolamine inefficient, pH-dependent Escherichia coli
phosphatidylglycerol
-
Escherichia coli
Phospholipids phosphatidylethanolamine/phosphatidylglycerol/cardiolipin, ratio 6/1/1 Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
plsB structural gene encoding glycerol 3-phosphate acyltransferase Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial inner membrane
-
Escherichia coli 5743
-
plasma membrane cytoplasmic side Escherichia coli 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not activated by divalent ions Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + sn-glycerol 3-phosphate Escherichia coli initial step in membrane phospholipid biosynthesis CoA + 1-acyl-sn-glycerol 3-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
overproducing strains VL3/pVL1, contains hybrid plasmid bearing plsB structural gene and VL3/plB3-4
-

Purification (Commentary)

Purification (Comment) Organism
Matrex Gel Red A, octyl-Sepharose CL-4B, hydroxylapatite Escherichia coli
solubilized, by non-ionic detergents Escherichia coli
solubilized, by non-ionic detergents and reconstituted in unilamellar phospholipid vesicles Escherichia coli

Renatured (Commentary)

Renatured (Comment) Organism
reconstituted in phospholipid vesicles containing phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglecerol in the approx. molar ratio of 6:1:1 Escherichia coli
reconstitution in cardiolipin/C12E8, i.e. non-ionic detergent, ratio 2:1 mixed micelles Escherichia coli
reconstitution in vesicles Escherichia coli

Storage Stability

Storage Stability Organism
-70°C, solubilized, indefinitely stable Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + sn-glycerol 3-phosphate dihydroxyacetone phosphate, ethylene glycol phosphate and 1,3-propanediol phosphate can replace glycerol 3-phosphate Escherichia coli CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
acyl-CoA + sn-glycerol 3-phosphate exclusively acylates glycerol 3-phosphate at the C-1-position Escherichia coli CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
acyl-CoA + sn-glycerol 3-phosphate initial step in membrane phospholipid biosynthesis Escherichia coli CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
acyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
Escherichia coli acyl-carrier protein + 1-acyl-sn-glycerol 3-phosphate
-
?
cis-vaccenoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
Escherichia coli acyl-carrier protein + 1-cis-vaccenoyl-sn-glycerol 3-phosphate
-
?
oleoyl-CoA + sn-glycerol 3-phosphate
-
Escherichia coli CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
?
palmitoyl-CoA + sn-glycerol 3-phosphate
-
Escherichia coli CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
?
palmitoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
Escherichia coli acyl-carrier protein + 1-palmitoyl-sn-glycerol 3-phosphate
-
?

Subunits

Subunits Comment Organism
?
-
Escherichia coli
More membranes of overproducing Escherichia coli strain VL3/pLB3-4 contain predominantly the dimeric form Escherichia coli