Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylethanolamine | seems to be required for optimal activity | Escherichia coli | |
Phospholipids | absolutely required | Escherichia coli | |
Phospholipids | bilayers required for activity: phosphatidylethanolamine/-glycerol, ratio 4/1, phosphatidylethanolamine/cardiolipin, ratio 4/1 | Escherichia coli |
General Stability | Organism |
---|---|
400 mM NaCl or KCl, 5 mM 2-mercaptoethanol, 5 mM MgCl2, stabilize membrane-bound or reconstituted enzyme | Escherichia coli |
glycerol, 20%, stabilizes | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cholate | phospholipids restore activity | Escherichia coli | |
deoxycholate | phospholipids restore activity | Escherichia coli | |
Detergents | e.g. Brij 35, Brij 58, Lubrol 17A-10, phospholipids restore activity | Escherichia coli | |
Triton X-100 | phospholipids restore activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.56 | - |
sn-glycerol 3-phosphate | strain 8 | Escherichia coli | |
3 | 3.2 | sn-glycerol 3-phosphate | strain BB26-36 | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Escherichia coli | 5886 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K-12 | - |
Escherichia coli | - |
K-12 derivative strains 8, wild-type genotype | - |
Escherichia coli | - |
A324 and 205 | - |
Escherichia coli | - |
overproducing mutants CV15, lsA-genotype and BB 26-36, plsB-genotype | - |
Escherichia coli 8 | - |
overproducing mutants CV15, lsA-genotype and BB 26-36, plsB-genotype | - |
Purification (Comment) | Organism |
---|---|
partial purification | Escherichia coli |
Renatured (Comment) | Organism |
---|---|
reconstitution in phospholipid bilayers | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.105 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | exclusively acylates glycerol 3-phosphate at the C-1-position | Escherichia coli | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | saturated acyl-CoAs preferred | Escherichia coli | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | exclusively acylates glycerol 3-phosphate at the C-1-position | Escherichia coli 8 | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | saturated acyl-CoAs preferred | Escherichia coli 8 | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
oleoyl-CoA + sn-glycerol 3-phosphate | 16% of the reaction rate with palmitoyl-CoA | Escherichia coli | CoA + 1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
oleoyl-CoA + sn-glycerol 3-phosphate | 16% of the reaction rate with palmitoyl-CoA | Escherichia coli 8 | CoA + 1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | 4fold better substrate than oleoyl-CoA | Escherichia coli | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | i.e. n-hexadecanoyl-CoA, best substrate | Escherichia coli | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | 4fold better substrate than oleoyl-CoA | Escherichia coli 8 | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | i.e. n-hexadecanoyl-CoA, best substrate | Escherichia coli 8 | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? |