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Literature summary for 2.3.1.15 extracted from

  • Snider, M.D.; Kennedy, E.P.
    Partial purification of glycerophosphate acyltransferase from Escherichia coli (1977), J. Bacteriol., 130, 1072-1083.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
phosphatidylethanolamine seems to be required for optimal activity Escherichia coli
Phospholipids absolutely required Escherichia coli
Phospholipids bilayers required for activity: phosphatidylethanolamine/-glycerol, ratio 4/1, phosphatidylethanolamine/cardiolipin, ratio 4/1 Escherichia coli

General Stability

General Stability Organism
400 mM NaCl or KCl, 5 mM 2-mercaptoethanol, 5 mM MgCl2, stabilize membrane-bound or reconstituted enzyme Escherichia coli
glycerol, 20%, stabilizes Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
cholate phospholipids restore activity Escherichia coli
deoxycholate phospholipids restore activity Escherichia coli
Detergents e.g. Brij 35, Brij 58, Lubrol 17A-10, phospholipids restore activity Escherichia coli
Triton X-100 phospholipids restore activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.56
-
sn-glycerol 3-phosphate strain 8 Escherichia coli
3 3.2 sn-glycerol 3-phosphate strain BB26-36 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
Escherichia coli 5886
-

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
K-12
-
Escherichia coli
-
K-12 derivative strains 8, wild-type genotype
-
Escherichia coli
-
A324 and 205
-
Escherichia coli
-
overproducing mutants CV15, lsA-genotype and BB 26-36, plsB-genotype
-
Escherichia coli 8
-
overproducing mutants CV15, lsA-genotype and BB 26-36, plsB-genotype
-

Purification (Commentary)

Purification (Comment) Organism
partial purification Escherichia coli

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution in phospholipid bilayers Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.105
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + sn-glycerol 3-phosphate exclusively acylates glycerol 3-phosphate at the C-1-position Escherichia coli CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
acyl-CoA + sn-glycerol 3-phosphate saturated acyl-CoAs preferred Escherichia coli CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
acyl-CoA + sn-glycerol 3-phosphate exclusively acylates glycerol 3-phosphate at the C-1-position Escherichia coli 8 CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
acyl-CoA + sn-glycerol 3-phosphate saturated acyl-CoAs preferred Escherichia coli 8 CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
oleoyl-CoA + sn-glycerol 3-phosphate 16% of the reaction rate with palmitoyl-CoA Escherichia coli CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
?
oleoyl-CoA + sn-glycerol 3-phosphate 16% of the reaction rate with palmitoyl-CoA Escherichia coli 8 CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
?
palmitoyl-CoA + sn-glycerol 3-phosphate 4fold better substrate than oleoyl-CoA Escherichia coli CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
?
palmitoyl-CoA + sn-glycerol 3-phosphate i.e. n-hexadecanoyl-CoA, best substrate Escherichia coli CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
?
palmitoyl-CoA + sn-glycerol 3-phosphate 4fold better substrate than oleoyl-CoA Escherichia coli 8 CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
?
palmitoyl-CoA + sn-glycerol 3-phosphate i.e. n-hexadecanoyl-CoA, best substrate Escherichia coli 8 CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
?