Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | not activated by 1,2-diacyl glycerol 3-phosphate | Escherichia coli | |
phosphatidylglycerol | 1.5 mM, 30-40fold activation of solubilized enzyme, inhibiton above | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cis-vaccenoyl-CoA | - |
Escherichia coli | |
phosphatidylethanolamine | 1,2-unsaturated species | Escherichia coli | |
phosphatidylglycerol | above 1.5 mM, activation below | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0056 | - |
palmitoyl-CoA | strain 8, membrane-associated enzyme | Escherichia coli | |
0.006 | - |
palmitoyl-CoA | strain VL3/pVL1 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, membrane-associated enzyme | Escherichia coli | |
0.0078 | - |
palmitoyl-CoA | strain VL3/pLB3-4 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, membrane-associated enzyme | Escherichia coli | |
0.102 | - |
sn-glycerol 3-phosphate | strain VL3/pVL1 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, membrane-associated enzyme | Escherichia coli | |
0.105 | - |
sn-glycerol 3-phosphate | strain 8, membrane-associated enzyme | Escherichia coli | |
0.108 | - |
sn-glycerol 3-phosphate | strain VL3/pLB3-4 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, membrane-associated enzyme | Escherichia coli | |
0.156 | - |
sn-glycerol 3-phosphate | membrane-bound enzyme | Escherichia coli | |
3 | - |
sn-glycerol 3-phosphate | solubilized enzyme in the presence of phosphatidylglycerol | Escherichia coli | |
3 | 3.2 | sn-glycerol 3-phosphate | reconstituted enzyme | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Escherichia coli | 5886 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
B | - |
Escherichia coli B / ATCC 11303 | - |
B | - |
Purification (Comment) | Organism |
---|---|
solubilized | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-vaccenoyl-CoA + sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + cis-vaccenoyl-sn-glycerol 3-phosphate | - |
? | |
cis-vaccenoyl-CoA + sn-glycerol 3-phosphate | - |
Escherichia coli B / ATCC 11303 | CoA + cis-vaccenoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | i.e. n-hexadecanoyl-CoA, best substrate | Escherichia coli | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | i.e. n-hexadecanoyl-CoA, best substrate | Escherichia coli B / ATCC 11303 | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? |