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Literature summary for 2.3.1.15 extracted from

  • Scheideler, M.A.; Bell, R.M.
    Characterization of active and latent forms of the membrane-associated sn-glycerol-3-phosphate acyltransferase of Escherichia coli (1991), J. Biol. Chem., 266, 14321-14327.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin activation at 1 mg/ml Escherichia coli
Phospholipids
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic cooperativity towards glycerol 3-phosphate and palmitoyl-CoA Escherichia coli
0.0046
-
palmitoyl-CoA strain 8, reconstituted enzyme Escherichia coli
0.0056
-
palmitoyl-CoA strain 8, membrane-associated enzyme Escherichia coli
0.006
-
palmitoyl-CoA strain VL3/pVL1 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, membrane-associated enzyme Escherichia coli
0.0078
-
palmitoyl-CoA strain VL3/pLB3-4 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, membrane-associated enzyme Escherichia coli
0.012
-
palmitoyl-CoA strain VL3/pVL1 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, reconstituted enzyme Escherichia coli
0.017
-
palmitoyl-CoA strain VL3/pLB3-4 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, reconstituted enzyme Escherichia coli
0.102
-
sn-glycerol 3-phosphate strain VL3/pVL1 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, membrane-associated enzyme Escherichia coli
0.105
-
sn-glycerol 3-phosphate strain 8, membrane-associated enzyme Escherichia coli
0.108
-
sn-glycerol 3-phosphate strain VL3/pLB3-4 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, membrane-associated enzyme Escherichia coli
0.79
-
sn-glycerol 3-phosphate strain 8, reconstituted enzyme Escherichia coli
0.92
-
sn-glycerol 3-phosphate strain VL3/pVL1 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, reconstituted enzyme Escherichia coli
1.1
-
sn-glycerol 3-phosphate strain VL3/pLB3-4 bearing plasmid which overproduces glycerol 3-phosphate acyltransferase, reconstituted enzyme Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
Escherichia coli 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
different molecular masses depending on Triton X-100 concentration Escherichia coli
88000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
overproducing strains VL3/pVL1, contains hybrid plasmid bearing plsB structural gene and VL3/plB3-4
-
Escherichia coli
-
K-12 derivative strains 8, wild-type genotype
-

Purification (Commentary)

Purification (Comment) Organism
partial purification Escherichia coli

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution in cardiolipin vesicles Escherichia coli
reconstitution in vesicles Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3 4 reconstituted enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmitoyl-CoA + sn-glycerol 3-phosphate
-
Escherichia coli CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
?

Subunits

Subunits Comment Organism
?
-
Escherichia coli
More monomer or oligomer, depending on Triton X-100 concentration Escherichia coli