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Literature summary for 2.3.1.15 extracted from

  • Kessels, J.M.M.; Van Den Bosch, H.
    Characterization of reconstituted partially purified glycerophosphate acyltansferase from Escherichia coli (1982), Biochim. Biophys. Acta, 713, 570-580.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phosphatidylglycerol
-
Escherichia coli
Phospholipids
-
Escherichia coli

General Stability

General Stability Organism
ion exchange chromatography, hydrophobic interaction chromatography, affinity chromatography, gel filtration and isoelectric focusing inactivate enzyme Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Triton X-100
-
Escherichia coli
Trypsin
-
Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane active site on outside surface Escherichia coli 5886
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + sn-glycerol 3-phosphate Escherichia coli
-
CoA + 1-acyl-sn-glycerol 3-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
K-12
-

Purification (Commentary)

Purification (Comment) Organism
partial purification Escherichia coli

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution in phosphatidylcholine/phosphatidylglycerol, ratio 4:1, vesicles, 90% of active site of the reconstituted enzyme are located at the outside surface Escherichia coli
reconstitution in vesicles Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.015
-
reconstituted enzyme Escherichia coli

Storage Stability

Storage Stability Organism
-40°C, solubilized enzyme, at least 1 year, no loss of activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + sn-glycerol 3-phosphate
-
Escherichia coli CoA + 1-acyl-sn-glycerol 3-phosphate
-
?
acyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
Escherichia coli acyl-carrier protein + 1-acyl-sn-glycerol 3-phosphate
-
?
palmitoyl-CoA + sn-glycerol 3-phosphate
-
Escherichia coli CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
reconstituted enzyme, 2 optima Escherichia coli
30 35 membrane-associated enzyme Escherichia coli
35
-
reconstituted enzyme, 2 optima Escherichia coli

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 50 approx. half-maximal activity at 10°C and 50°C Escherichia coli
20 40 approx. half-maximal activity at 20°C and about 70% of maximal activity at 40°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.2
-
reconstituted enzyme Escherichia coli
9.5
-
membrane-associated enzyme Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.2 approx. half-maximal activity at pH 7.5 and 9.2, reconstituted enzyme Escherichia coli
8 9.5 approx. half-maximal activity at pH 8.0 and 9.5, membrane-associated enzyme Escherichia coli