Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylglycerol | - |
Escherichia coli | |
Phospholipids | - |
Escherichia coli |
General Stability | Organism |
---|---|
ion exchange chromatography, hydrophobic interaction chromatography, affinity chromatography, gel filtration and isoelectric focusing inactivate enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | - |
Escherichia coli | |
Trypsin | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | active site on outside surface | Escherichia coli | 5886 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | Escherichia coli | - |
CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K-12 | - |
Purification (Comment) | Organism |
---|---|
partial purification | Escherichia coli |
Renatured (Comment) | Organism |
---|---|
reconstitution in phosphatidylcholine/phosphatidylglycerol, ratio 4:1, vesicles, 90% of active site of the reconstituted enzyme are located at the outside surface | Escherichia coli |
reconstitution in vesicles | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.015 | - |
reconstituted enzyme | Escherichia coli |
Storage Stability | Organism |
---|---|
-40°C, solubilized enzyme, at least 1 year, no loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-[acyl-carrier protein] + sn-glycerol 3-phosphate | - |
Escherichia coli | acyl-carrier protein + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
reconstituted enzyme, 2 optima | Escherichia coli |
30 | 35 | membrane-associated enzyme | Escherichia coli |
35 | - |
reconstituted enzyme, 2 optima | Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 50 | approx. half-maximal activity at 10°C and 50°C | Escherichia coli |
20 | 40 | approx. half-maximal activity at 20°C and about 70% of maximal activity at 40°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.2 | - |
reconstituted enzyme | Escherichia coli |
9.5 | - |
membrane-associated enzyme | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.2 | approx. half-maximal activity at pH 7.5 and 9.2, reconstituted enzyme | Escherichia coli |
8 | 9.5 | approx. half-maximal activity at pH 8.0 and 9.5, membrane-associated enzyme | Escherichia coli |