Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | i.e. octylphenoxy polyethoxyethanol, slight stimulation at 0.1 mg/ml | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
deoxycholate | above 0.35 mg/ml | Saccharomyces cerevisiae | |
dihydroxyacetone phosphate | - |
Saccharomyces cerevisiae | |
N-ethylmaleimide | 0.4 mM, 60% inhibition after 10 min | Saccharomyces cerevisiae | |
palmitoyl-CoA | 0.08 mM | Saccharomyces cerevisiae | |
Triton X-100 | strong, above 0.15 mg/ml | Saccharomyces cerevisiae | |
Trypsin | 0.06 mg/ml, 80% inhibition after 10 min | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
sn-glycerol 3-phosphate | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
S288c | - |
Storage Stability | Organism |
---|---|
-15°C, 3-5 mg/ml protein, membrane preparation, at least 2 months | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | enzyme has both 3-glycerophosphate acyltransferase and dihydroxyacetonephosphate acyltransferase activity | Saccharomyces cerevisiae | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
myristoyl-CoA + sn-glycerol 3-phosphate | i.e. tetradecanoyl-CoA | Saccharomyces cerevisiae | CoA + 1-myristoyl-sn-glycerol 3-phosphate | - |
? | |
n-dodecanoyl-CoA + sn-glycerol 3-phosphate | i.e. lauroyl-CoA, poor substrate | Saccharomyces cerevisiae | CoA + 1-n-dodecanoyl-sn-glycerol 3-phosphate | - |
? | |
oleoyl-CoA + sn-glycerol 3-phosphate | - |
Saccharomyces cerevisiae | CoA + 1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | i.e. n-hexadecanoyl-CoA, best substrate | Saccharomyces cerevisiae | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme may be identical with EC 2.3.1.42 | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | 7 | - |
Saccharomyces cerevisiae |
7 | - |
50% of maximal activity at pH 7.4 | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.6 | 8.9 | maximal activity at pH 6.6, approx. 30% of maximal activity at pH 8.9 | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.54 | - |
dihydroxyacetone phosphate | - |
Saccharomyces cerevisiae |