KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
anthranilate | - |
Dianthus caryophyllus | |
0.05 | - |
benzoyl-CoA | - |
Dianthus caryophyllus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-CoA + anthranilate | Dianthus caryophyllus | cinnamoyl-CoA, salicyl-CoA and 4-coumaroyl-CoA can act as donors with 73%, 70% and 65% efficiency respectively, enzyme is involved in the biosynthesis of phytoalexins, 4-hydroxyanthranilate is the most probable substrate in vivo | CoA + N-benzoylanthranilate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dianthus caryophyllus | - |
enzyme is induced by addition of crude elicitor from the cell walls of Phythophthora megasperma f. sp. glycinea or by commercial yeast extract | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Q-Sepharose, partially purified | Dianthus caryophyllus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
callus | cell culture | Dianthus caryophyllus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-CoA + 3-hydroxyanthranilate | 20% of activity with anthranilate | Dianthus caryophyllus | CoA + N-benzoyl-3-hydroxyanthranilate | - |
? | |
benzoyl-CoA + 4-hydroxyanthranilate | 10% of activity with anthranilate | Dianthus caryophyllus | CoA + N-benzoyl-4-hydroxyanthranilate | - |
? | |
benzoyl-CoA + anthranilate | cinnamoyl-CoA, salicyl-CoA and 4-coumaroyl-CoA can act as donors | Dianthus caryophyllus | CoA + N-benzoylanthranilate | - |
? | |
benzoyl-CoA + anthranilate | cinnamoyl-CoA, salicyl-CoA and 4-coumaroyl-CoA can act as donors with 73%, 70% and 65% efficiency respectively, enzyme is involved in the biosynthesis of phytoalexins, 4-hydroxyanthranilate is the most probable substrate in vivo | Dianthus caryophyllus | CoA + N-benzoylanthranilate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Dianthus caryophyllus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
crude extract, 5 min, pH 7.5, significant loss of activity | Dianthus caryophyllus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
true pH-optimum may be closer to the amino-pK of anthranilate at 4.95 | Dianthus caryophyllus |
6.8 | 7 | crude extract, assay at pH 7.0 | Dianthus caryophyllus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.8 | - |
Dianthus caryophyllus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
crude extract, 4°C, almost all activity irreversibly lost within less than 2 h | Dianthus caryophyllus |
7 | - |
very labile at pH below | Dianthus caryophyllus |