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Literature summary for 2.3.1.133 extracted from
- Characterization of hydroxycinnamoyltransferase from rice and its application for biological synthesis of hydroxycinnamoyl glycerols (2012), Phytochemistry, 76, 25-31.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene OsHCT4, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as soluble GST-tagged enzyme using vector pGEX 5X-1 in Escherichia coli strain BL21(DE3) | Oryza sativa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0601 | - |
4-coumaroyl-CoA | pH 7.5, 37°C, with glycerol | Oryza sativa |  |
| 0.0721 | - |
feruloyl-CoA | pH 7.5, 37°C, with glycerol | Oryza sativa | |
| 0.0859 | - |
glycerol | pH 7.5, 37°C, with feruloyl-CoA | Oryza sativa | |
| 0.102 | - |
caffeoyl-CoA | pH 7.5, 37°C, with glycerol | Oryza sativa | |
| 0.1558 | - |
shikimate | pH 7.5, 37°C, with feruloyl-CoA | Oryza sativa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Oryza sativa | no determination of the in vivo substrate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
gene OsHCT4 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| soluble recombinant GST-tagged OsHCT4 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography | Oryza sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-coumaroyl-CoA + glycerol | - |
Oryza sativa | CoA + 2-O-(4-coumaroyl)glycerol + 1-O-(4-coumaroyl)glycerol | - |
? | |
| 4-coumaroyl-CoA + shikimate | - |
Oryza sativa | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
| caffeoyl-CoA + glycerol | - |
Oryza sativa | CoA + ? | - |
? | |
| caffeoyl-CoA + shikimate | - |
Oryza sativa | CoA + 5-O-caffeoylshikimate | - |
? | |
| feruloyl-CoA + glycerol | - |
Oryza sativa | CoA + ? | - |
? | |
| feruloyl-CoA + shikimate | - |
Oryza sativa | CoA + 5-O-feruloylshikimate | - |
? | |
| additional information | no determination of the in vivo substrate | Oryza sativa | ? | - |
? | |
| additional information | OsHCT4 mediates the trans-esterification of glycerol as well as shikimic acid in the presence of hydroxycinnamoyl-CoA in vitro, reaction product structure determination by NMR | Oryza sativa | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HCS/QT | - |
Oryza sativa |
| hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyltransferase | - |
Oryza sativa |
| OsHCT4 | - |
Oryza sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Oryza sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Oryza sativa |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | OsHCT1 and OsHCT2 are members of the HCS/QT family, while OsHCT3 and OsHCT4 belong to a distinct group. OsHCT3 and OsHCT4 may employ a different acyl group acceptor | Oryza sativa |
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Release 2023.1 (January 2023)
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