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Literature summary for 2.3.1.133 extracted from

  • Sullivan, M.
    A novel red clover hydroxycinnamoyl transferase has enzymatic activities consistent with a role in phaselic acid biosynthesis (2009), Plant Physiol., 150, 1866-1879.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.133

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Trifolium pratense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48300
-
 calculated from cDNA Trifolium pratense

Organism

Organism UniProt Comment Textmining
Trifolium pratense B6D7P1 cDNA HCT1A (almost identical to HCT1B)
-
Trifolium pratense B6D7P2
-
-
Trifolium pratense B6DQK4 cDNA HCT1B (almost identical to HCT1A)
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf expression analyses indicate that HCT1 mRNA accumulates to 4fold higher levels in stems than in leaves Trifolium pratense
-
leaf HCT2 mRNA accumulates to 10fold higher levels in leaves than in stems Trifolium pratense
-
stem expression analyses indicate that HCT1 mRNA accumulates to 4fold higher levels in stems than in leaves Trifolium pratense
-
stem HCT2 mRNA accumulates to 10fold higher levels in leaves than in stems Trifolium pratense
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaroyl-CoA + L-malate
-
 Trifolium pratense CoA + 2-O-(4-coumaryl)-L-malate
-
 ?
4-coumaroyl-CoA + quinate reaction products are detected when quinic acid is used as an acceptor, although the amount of product formed is less than 2% of that formed when shikimic acid serves as the acceptor Trifolium pratense CoA + 4-coumaroylquinate
-
 ?
4-coumaroyl-CoA + shikimate
-
 Trifolium pratense CoA + 5-O-(4-coumaroyl)shikimate
-
 ?
caffeoyl-CoA + L-malate
-
 Trifolium pratense CoA + 2-O-caffeoyl-L-malate
-
 ?
caffeoyl-CoA + quinate reaction products are detected when quinic acid is used as an acceptor, although the amount of product formed is less than 2% of that formed when shikimic acid serves as the acceptor Trifolium pratense CoA + caffeoylquinate
-
 ?
caffeoyl-CoA + shikimate
-
 Trifolium pratense CoA + 5-O-caffeoylshikimate
-
 ?
additional information no reaction products are detected when HCT1 and either hydroxycinnamoyl-CoA derivative are incubated with malic acid, Glc, or L-DOPA as acceptor Trifolium pratense ?
-
 ?
additional information no reaction products are detected when shikimic acid, quinic acid, Glc, or L-DOPA is used as an acceptor Trifolium pratense ?
-
 ?

Synonyms

Synonyms Comment Organism
HCT1 two cDNAs are isolated HCT1A and HCT1B which are nearly identical Trifolium pratense
HCT2
-
 Trifolium pratense
hydroxycinnamoyltransferase
-
 Trifolium pratense

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Trifolium pratense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Trifolium pratense

pH Range

pH Minimum pH Maximum Comment Organism
6.1 6.7 optimal pH range with malate as the acceptor, highest reaction rates are observed for both hydroxycinnamoyl-CoA derivatives between pH 6.1 and 6.7 using sodium phosphate buffer Trifolium pratense
7.5 7.9 optimal pH range with shikimic acid as the acceptor, highest reaction rates are observed for both hydroxycinnamoyl-CoA derivatives between pH 7.5 and 7.9 using sodium phosphate buffer Trifolium pratense