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Literature summary for 2.3.1.129 extracted from

  • Jenkins, R.J.; Dotson, G.D.
    A continuous fluorescent enzyme assay for early steps of lipid A biosynthesis (2012), Anal. Biochem., 425, 21-27.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.129

Application

Application Comment Organism
drug development Lpxa is a viable target for antimicrobial drug development Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
gene lpxA, cloning from strain strain MG1655, subcloning in strain XL-1 Blue, expression in Escherichia coli strain BL21-AI/pET24a Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten steady-state kinetics of LpxA, overview Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine Escherichia coli
-
 an [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 strain MG1655, gene lpxA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant LpxA from Echerichia coli strain BL21-AI/pET24a by Reactive Green 19 affinity chromatography, dialysis, and ion exchange chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
an (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine
-
 Escherichia coli an [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
-
 ?
an (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine assay is optimal at 10 nM LpxA Escherichia coli an [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
-
 ?

Synonyms

Synonyms Comment Organism
LpxA
-
 Escherichia coli
type II ACP-dependent UDP-N-acetylglucosamine acyltransferase
-
 Escherichia coli
type II acyl carrier protein-dependent UDP-N-acetylglucosamine acyltransferase
-
 Escherichia coli
UDP-N-acetylglucosamine acyltransferase
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Escherichia coli

General Information

General Information Comment Organism
evolution the enzymes in early lipid A biosynthesis are highly conserved in gram-negative pathogens. LpxA and LpxD are structurally homologous possessing the unique left-handed beta-helix fold, which stems from an extensive hexapeptide repeat motif in their respective primary amino acid sequences Escherichia coli
metabolism UDP-N-acetylglucosamine acyltransferase, LpxA, catalyzes the first step of lipid A biosynthesis Escherichia coli
physiological function UDP-GlcNAc is acylated at the 3-hydroxyl through a thermodynamically unfavorable reaction catalyzed by the type II acyl carrier protein-dependent UDP-N-acetylglucosamine acyltransferase, LpxA, first step in biosynthesis of lipid A and essential for survival among Gram-negative bacteria that synthesize lipopolysaccharide. Lipopolysaccharide contains the glycolipid lipid A, which anchors it to the membrane through fatty acyl chains, a core polysaccharide region, and an O-antigen repeat. Lipid A is an essential moiety necessary for survival of the bacterium and further plays a crucial role in natural antibacterial resistance and bacterial sepsis Escherichia coli