Literature summary for 2.3.1.129 extracted from

Robins, L.I.; Williams, A.H.; Raetz, C.R.
Structural basis for the sugar nucleotide and acyl-chain selectivity of Leptospira interrogans LpxA (2009), Biochemistry, 48, 6191-6201.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Leptospira interrogans

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structures of free LiLpxA and its complexes with its product UDP-3-N-((R)-3-hydroxylauroyl)-GlcNAc3N and with its substrate (R)-3-hydroxylauroyl-methylphosphopantetheine are presented. The selectivity of LiLpxA for UDPGlcNAc3N may be explained by the orientation of the backbone carbonyl group of Q68, which differs by 82° from the corresponding Q73 carbonyl group in Escherichia coli LpxA. This arrangement provides an extra hydrogen-bond acceptor for the 3-NH2 group of UDP-GlcNAc3N in LiLpxA. The R-3-hydroxylauroyl selectivity of LiLpxA is explained by the position of the K171 side chain, which limits the length of the acyl-chain-binding groove. H120 functions as a catalytic base	Leptospira interrogans

Crystallization (Comment)

Organism

crystal structures of free LiLpxA and its complexes with its product UDP-3-N-((R)-3-hydroxylauroyl)-GlcNAc3N and with its substrate (R)-3-hydroxylauroyl-methylphosphopantetheine are presented. The selectivity of LiLpxA for UDPGlcNAc3N may be explained by the orientation of the backbone carbonyl group of Q68, which differs by 82° from the corresponding Q73 carbonyl group in Escherichia coli LpxA. This arrangement provides an extra hydrogen-bond acceptor for the 3-NH2 group of UDP-GlcNAc3N in LiLpxA. The R-3-hydroxylauroyl selectivity of LiLpxA is explained by the position of the K171 side chain, which limits the length of the acyl-chain-binding groove. H120 functions as a catalytic base

Leptospira interrogans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.004	-	3R-3-hydroxylauroyl-methylphosphopantetheine	-	Leptospira interrogans

Organism

Organism	UniProt	Comment	Textmining
Leptospira interrogans	Q8EZA6	-	-

Purification (Commentary)

Purification (Comment)	Organism
ion-exchange-chromatography and gel filtration	Leptospira interrogans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3	-	using synthetic (3R)-3-hydroxylauroyl-methylphosphopantetheine + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose as a substrate the specific activity is comparable to substrates (3R)-3-hydroxylauroyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose	Leptospira interrogans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(3R)-3-hydroxylauroyl-methylphosphopantetheine + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose	synthetic (3R)-3-hydroxylauroyl-methylphosphopantetheine is an excellent alternative donor substrate for LiLpxA	Leptospira interrogans	methylphosphopantetheine + UDP-3-N-((3R)-3-hydroxylauroyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose	-	?
(R)-3-hydroxylauroyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose	LiLpxA is highly selective for (R)-3-hydroxylauroyl-[acyl-carrier protein] and UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose	Leptospira interrogans	[acyl-carrier protein] + UDP-3-N-((3R)-3-hydroxylauroyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose	-	?

Subunits

Subunits	Comment	Organism
homotrimer	in aqueous solution, shown by gel filtration	Leptospira interrogans

Synonyms

Synonyms	Comment	Organism
LiLpxA	-	Leptospira interrogans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Leptospira interrogans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Leptospira interrogans