Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | 0.1 mM, complete inhibition, activity can be restored by incubation with 2 mM dithiothreitol for 30 min at 0°C | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
3,4-dichloroaniline | - |
Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
1 * 47000, SDS-PAGE | Glycine max |
48000 | - |
gel filtration | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, ion-exchange, Sephadex G-100, hydrophobic interaction, gel filtration | Glycine max |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.143 | - |
- |
Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + 2,3,4,5-tetrachloroaniline | 29% of activity with 3,4-dichloroaniline | Glycine max | CoA + N-(2,3,4,5-tetrachlorophenyl)-malonamate | - |
? | |
malonyl-CoA + 2,3,4-trichloroaniline | 50% of activity with 3,4-dichloroaniline | Glycine max | CoA + N-(2,3,4-trichlorophenyl)-malonamate | - |
? | |
malonyl-CoA + 2,4,5-trichloroaniline | 22% of activity with 3,4-dichloroaniline | Glycine max | CoA + N-(2,4,5-trichlorophenyl)-malonamate | - |
? | |
malonyl-CoA + 2,4-dichloroaniline | 53% of activity with 3,4-dichloroaniline | Glycine max | CoA + N-(2,4-dichlorophenyl)-malonamate | - |
? | |
malonyl-CoA + 2,5-dichloroaniline | 53% of activity with 3,4-dichloroaniline | Glycine max | CoA + N-(2,5-dichlorophenyl)-malonamate | - |
? | |
malonyl-CoA + 2-chloroaniline | 61% of activity with 3,4-dichloroaniline | Glycine max | CoA + N-(2-chlorophenyl)-malonamate | - |
? | |
malonyl-CoA + 3,4,5-trichloroaniline | 72% of activity with 3,4-dichloroaniline | Glycine max | CoA + N-(3,4,5-trichlorophenyl)-malonamate | - |
? | |
malonyl-CoA + 3,4,5-trichloroaniline | 30% of activity with 3,4-dichloroaniline | Glycine max | CoA + N-(3,4,5-trichloro-phenyl)-malonamate | - |
? | |
malonyl-CoA + 3,4-dichloroaniline | preferred substrate | Glycine max | CoA + N-(3,4-dichlorophenyl)-malonamate | - |
? | |
malonyl-CoA + 3,5-dichloroaniline | 48% of activity with 3,4-dichloroaniline | Glycine max | CoA + N-(3,5-dichlorophenyl)-malonamate | - |
? | |
malonyl-CoA + 3-chloroaniline | 67% of activity with 3,4-dichloroaniline | Glycine max | CoA + N-(3-chlorophenyl)-malonamate | - |
? | |
malonyl-CoA + 4-aminophenol | 20% of activity with 3,4-dichloroaniline | Glycine max | CoA + N-(phenyl)-malonamate | - |
? | |
malonyl-CoA + 4-bromoaniline | 75% of activity with 3,4-dichloroaniline | Glycine max | CoA + N-(4-bromophenyl)-malonamate | - |
? | |
malonyl-CoA + 4-chloroaniline | 87% of activity with 3,4-dichloroaniline | Glycine max | CoA + N-(4-chlorophenyl)-malonamate | - |
? | |
malonyl-CoA + 4-nitroaniline | 43% of activity with 3,4-dichloroaniline | Glycine max | CoA + N-(4-nitrophenyl)-malonamate | - |
? | |
malonyl-CoA + 7-amino-4-methylcoumarin | 70% of activity with 3,4-dichloroaniline | Glycine max | N-(4-methylcoumaryl)-7-malonamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 47000, SDS-PAGE | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | 7.2 | - |
Glycine max |