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Literature summary for 2.3.1.1 extracted from

  • Sonoda, T.; Tatibana, M.
    Purification of N-acetyl-L-glutamate synthetase from rat liver mitochondria and substrate and activator specificity of the enzyme (1983), J. Biol. Chem., 258, 9839-9844.
    View publication on PubMed
Search for more literature for 2.3.1.1

Activating Compound

Activating Compound Comment Organism Structure
arginine 5.1fold activiation of matrix enzyme Rattus norvegicus
clupein
-
 Rattus norvegicus
L-argininic acid activates to a lesser extent than L-arginine Rattus norvegicus
Polyarginine
-
 Rattus norvegicus
polylysine
-
 Rattus norvegicus
salmin
-
 Rattus norvegicus

General Stability

General Stability Organism
Triton X-100 stabilizes Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.76
-
 acetyl-CoA
-
 Rattus norvegicus
3.7
-
 L-glutamate
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 3 * 57000, SDS-PAGE Rattus norvegicus
160000
-
 gel filtration, sucrose density gradient centrifugation Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE Biogel, Sephacryl S-200, aminoacetyl biogel A, affi-gel blue, sucrose gradient, isoelectric focusing Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
92.4
-
 in the presence of 1 mM L-arginine Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + DL-2-aminopimelate
-
 Rattus norvegicus CoA + 2-acetylaminoheptanedioate
-
 ?
acetyl-CoA + glycine
-
 Rattus norvegicus CoA + acetylaminoacetate
-
 ?
acetyl-CoA + L-2-aminoadipate DL-2-aminoadipate, 5.2% of activity with L-glutamate Rattus norvegicus CoA + 2-acetylaminohexanedioate
-
 ?
acetyl-CoA + L-glutamate
-
 Rattus norvegicus CoA + N-acetyl-L-glutamate
-
 ?
acetyl-CoA + L-glutamate-gamma-hydroxamate 15.5% of activity with glutamate Rattus norvegicus CoA + N-acetyl-L-glutamate-gamma-hydroxamate
-
 ?
acetyl-CoA + L-glutamine
-
 Rattus norvegicus ?
-
 ?
propionyl-CoA + L-glutamate
-
 Rattus norvegicus CoA + N-propionyl-L-glutamate
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 57000, SDS-PAGE Rattus norvegicus