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Literature summary for 2.3.1.1 extracted from

  • Powers-Lee, S.G.
    N-Acetylglutamate synthase (1985), Methods Enzymol., 113, 27-35.
    View publication on PubMed
Search for more literature for 2.3.1.1

Activating Compound

Activating Compound Comment Organism Structure
arginine
-
 Homo sapiens
arginine 0.02 mM, 4.2fold activation Rattus norvegicus
Cationic polypeptides 4fold activation Rattus norvegicus
Triton X-100 0.1%, 4fold activation Rattus norvegicus

General Stability

General Stability Organism
Triton X-100, 0.1%, stabilizes liver enzyme Rattus norvegicus
use of silicone-treated glassware or plastic tubes, e.g. polyethylene, polycarbonate or polypropylene stabilizes the enzyme Salmonella enterica subsp. enterica serovar Typhimurium
use of silicone-treated glassware or plastic tubes, e.g. polyethylene, polycarbonate or polypropylene stabilizes the enzyme Escherichia coli
use of silicone-treated glassware or plastic tubes, e.g. polyethylene, polycarbonate or polypropylene stabilizes the enzyme Homo sapiens
use of silicone-treated glassware or plastic tubes, e.g. polyethylene, polycarbonate or polypropylene stabilizes the enzyme Rattus norvegicus
use of silicone-treated glassware or plastic tubes, e.g. polyethylene, polycarbonate or polypropylene stabilizes the enzyme Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
coenzyme A 2.5 mM, 50% inhibition Escherichia coli
coenzyme A 1 mM, 20% inhibition Rattus norvegicus
high ionic strength
-
 Escherichia coli
high ionic strength
-
 Rattus norvegicus
N-acetylglutamate
-
 Escherichia coli
N-acetylglutamate 2 mM, 88% inhibition Rattus norvegicus
propionyl-CoA
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.76
-
 acetyl-CoA
-
 Rattus norvegicus
3.7
-
 L-glutamate
-
 Rattus norvegicus
4.6
-
 acetyl-CoA
-
 Salmonella enterica subsp. enterica serovar Typhimurium
4.7
-
 L-glutamate partially purified enzyme Homo sapiens
8.1
-
 acetyl-CoA partially purified enzyme Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 3 * 57000, SDS-PAGE Rattus norvegicus
160000
-
 gel filtration, sucrose density gradient centrifugation Rattus norvegicus
190000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + L-glutamate Rattus norvegicus
-
 CoA + N-acetyl-L-glutamate
-
 ?
acetyl-CoA + L-glutamate Salmonella enterica subsp. enterica serovar Typhimurium enzyme catalyzes the first step in the biosynthesis of arginine CoA + N-acetyl-L-glutamate
-
 ?
acetyl-CoA + L-glutamate Escherichia coli enzyme catalyzes the first step in the biosynthesis of arginine CoA + N-acetyl-L-glutamate
-
 ?
acetyl-CoA + L-glutamate Pseudomonas aeruginosa enzyme catalyzes the first step in the biosynthesis of arginine CoA + N-acetyl-L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Homo sapiens
-
-
-
Pseudomonas aeruginosa
-
-
-
Rattus norvegicus
-
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE Biogel, Sephacryl S-200, aminoacetyl biogel A, affi-gel blue, sucrose gradient, isoelectric focusing Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-
liver
-
 Rattus norvegicus
-
small intestine
-
 Homo sapiens
-
small intestine mucosa Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000014
-
-
 Rattus norvegicus
0.00013
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium
0.00013
-
-
 Escherichia coli
0.000268
-
 activity in liver of adult Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + DL-2-aminopimelate
-
 Escherichia coli CoA + 2-acetylaminoheptanedioate
-
 ?
acetyl-CoA + DL-2-aminopimelate 5.2% of activity with L-glutamate Rattus norvegicus CoA + 2-acetylaminoheptanedioate
-
 ?
acetyl-CoA + glycine 2.9% of activity with L-glutamate Rattus norvegicus CoA + acetylaminoacetate
-
 ?
acetyl-CoA + L-2-aminoadipate
-
 Rattus norvegicus CoA + 2-acetylaminohexanedioate
-
 ?
acetyl-CoA + L-2-aminoadipate 1.3% of activity with L-glutamate Escherichia coli CoA + 2-acetylaminohexanedioate
-
 ?
acetyl-CoA + L-glutamate
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + N-acetyl-L-glutamate
-
 ?
acetyl-CoA + L-glutamate
-
 Escherichia coli CoA + N-acetyl-L-glutamate
-
 ?
acetyl-CoA + L-glutamate
-
 Homo sapiens CoA + N-acetyl-L-glutamate
-
 ?
acetyl-CoA + L-glutamate
-
 Rattus norvegicus CoA + N-acetyl-L-glutamate
-
 ?
acetyl-CoA + L-glutamate
-
 Pseudomonas aeruginosa CoA + N-acetyl-L-glutamate
-
 ?
acetyl-CoA + L-glutamate enzyme catalyzes the first step in the biosynthesis of arginine Salmonella enterica subsp. enterica serovar Typhimurium CoA + N-acetyl-L-glutamate
-
 ?
acetyl-CoA + L-glutamate enzyme catalyzes the first step in the biosynthesis of arginine Escherichia coli CoA + N-acetyl-L-glutamate
-
 ?
acetyl-CoA + L-glutamate enzyme catalyzes the first step in the biosynthesis of arginine Pseudomonas aeruginosa CoA + N-acetyl-L-glutamate
-
 ?
acetyl-CoA + L-glutamine 5% of activity with acetyl-CoA Escherichia coli ?
-
 ?
acetyl-CoA + L-glutamine 6.8% of activity with L-glutamate Rattus norvegicus ?
-
 ?
propionyl-CoA + L-glutamate 4% of activity with acetyl-CoA Escherichia coli CoA + N-propionyl-L-glutamate
-
 ?
propionyl-CoA + L-glutamate 4.3% of activity with acetyl-CoA Rattus norvegicus CoA + N-propionyl-L-glutamate
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 57000, SDS-PAGE Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 liver enzyme, in the absence of arginine Rattus norvegicus
8.5
-
-
 Rattus norvegicus
8.5
-
 liver enzyme, in the presence of arginine Homo sapiens
10
-
-
 Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 highest stability Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.71
-
 propionyl-CoA
-
 Rattus norvegicus