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Literature summary for 2.2.1.7 extracted from

  • Battistini, M.R.; Shoji, C.; Handa, S.; Breydo, L.; Merkler, D.J.
    Mechanistic binding insights for 1-deoxy-D-xylulose-5-phosphate synthase, the enzyme catalyzing the first reaction of isoprenoid biosynthesis in the malaria-causing protists, Plasmodium falciparum and Plasmodium vivax (2016), Protein Expr. Purif., 120, 16-27.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.2.1.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Plasmodium falciparum
expressed in Escherichia coli BL21(DE3) cells Plasmodium vivax

Inhibitors

Inhibitors Comment Organism Structure
3-fluoro-2-oxopropanoate competitive inhibition with respect to pyruvate Plasmodium falciparum
3-fluoro-2-oxopropanoate competitive inhibition with respect to pyruvate Plasmodium vivax
Methylacetylphosphonate competitive inhibition with respect to pyruvate Plasmodium falciparum
Methylacetylphosphonate competitive inhibition with respect to pyruvate Plasmodium vivax

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 D-glyceraldehyde 3-phosphate apparent value, at pH 7.0 and 37°C Plasmodium falciparum
0.026
-
 D-glyceraldehyde 3-phosphate apparent value, at pH 7.0 and 37°C Plasmodium vivax
0.64
-
 pyruvate apparent value, at pH 7.0 and 37°C Plasmodium falciparum
1.09
-
 pyruvate apparent value, at pH 7.0 and 37°C Plasmodium vivax

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
105800
-
 x * 105800, estimated from SDS-PAGE Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate + D-glyceraldehyde 3-phosphate Plasmodium falciparum
-
 1-deoxy-D-xylulose 5-phosphate + CO2
-
 ?
pyruvate + D-glyceraldehyde 3-phosphate Plasmodium vivax
-
 1-deoxy-D-xylulose 5-phosphate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-
Plasmodium vivax
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex gel filtration Plasmodium falciparum
Ni-NTA column chromatography and Superdex gel filtration Plasmodium vivax

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + D-glyceraldehyde 3-phosphate
-
 Plasmodium falciparum 1-deoxy-D-xylulose 5-phosphate + CO2
-
 ?
pyruvate + D-glyceraldehyde 3-phosphate
-
 Plasmodium vivax 1-deoxy-D-xylulose 5-phosphate + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 105800, estimated from SDS-PAGE Plasmodium falciparum

Synonyms

Synonyms Comment Organism
DXS
-
 Plasmodium falciparum
DXS
-
 Plasmodium vivax

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1
-
 D-glyceraldehyde 3-phosphate apparent value, at pH 7.0 and 37°C Plasmodium falciparum
4.2
-
 pyruvate apparent value, at pH 7.0 and 37°C Plasmodium falciparum
6.4
-
 D-glyceraldehyde 3-phosphate apparent value, at pH 7.0 and 37°C Plasmodium vivax
6.6
-
 pyruvate apparent value, at pH 7.0 and 37°C Plasmodium vivax

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate
-
 Plasmodium falciparum
thiamine diphosphate
-
 Plasmodium vivax

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.035
-
 3-fluoro-2-oxopropanoate apparent value, at pH 7.0 and 37°C Plasmodium vivax
0.043
-
 3-fluoro-2-oxopropanoate apparent value, at pH 7.0 and 37°C Plasmodium falciparum
0.046
-
 Methylacetylphosphonate apparent value, at pH 7.0 and 37°C Plasmodium falciparum
0.075
-
 Methylacetylphosphonate apparent value, at pH 7.0 and 37°C Plasmodium vivax

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1
-
 D-glyceraldehyde 3-phosphate apparent value, at pH 7.0 and 37°C Plasmodium falciparum
6.5
-
 pyruvate apparent value, at pH 7.0 and 37°C Plasmodium falciparum
240
-
 D-glyceraldehyde 3-phosphate apparent value, at pH 7.0 and 37°C Plasmodium vivax
250
-
 pyruvate apparent value, at pH 7.0 and 37°C Plasmodium vivax