Literature summary for 2.2.1.7 extracted from

Battilana, J.; Emanuelli, F.; Gambino, G.; Gribaudo, I.; Gasperi, F.; Boss, P.K.; Grando, M.S.
Functional effect of grapevine 1-deoxy-D-xylulose 5-phosphate synthase substitution K284N on Muscat flavour formation (2011), J. Exp. Bot., 62, 5497-5508.

Search for more literature for 2.2.1.7

Application

Application	Comment	Organism
agriculture	single nucleotide polymorphism K284N is significantly associated with Muscat-flavoured varieties. Substitution influences the enzyme kinetics by increasing the catalytic efficiency and also dramatically affects monoterpene levels upon heterologous expression	Vitis vinifera

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Nicotiana tabacum	Vitis vinifera

Protein Variants

Protein Variants	Comment	Organism
K284N	single nucleotide polymorphism, significantly associated with Muscat-flavoured varieties. Substitution influences the enzyme kinetics by increasing the catalytic efficiency and also dramatically affects monoterpene levels upon heterologous expression	Vitis vinifera

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.585	-	pyruvate	mutant K284N, pH 8.0, 37°C	Vitis vinifera
0.661	-	pyruvate	wild-type, pH 8.0, 37°C	Vitis vinifera
1.67	-	D-glyceraldehyde 3-phosphate	mutant K284N, pH 8.0, 37°C	Vitis vinifera
1.78	-	D-glyceraldehyde 3-phosphate	wild-type, pH 8.0, 37°C	Vitis vinifera

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
77000	-	x * 77000, SDS-PAGE	Vitis vinifera

Organism

Organism	UniProt	Comment	Textmining
Vitis vinifera	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pyruvate + D-glyceraldehyde 3-phosphate	-	Vitis vinifera	1-deoxy-D-xylulose 5-phosphate + CO2	-	?

Subunits

Subunits	Comment	Organism
?	x * 77000, SDS-PAGE	Vitis vinifera

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Vitis vinifera

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Vitis vinifera

Expression

Organism	Comment	Expression
Vitis vinifera	correlation exists between the transcript profile and the accumulation of free monoterpenol odorants. The modulation of enzyme gene expression during berry development appears to be independent of nucleotide variation in the coding sequence	additional information

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
110	-	pyruvate	wild-type, pH 8.0, 37°C	Vitis vinifera
210	-	pyruvate	mutant K284N, pH 8.0, 37°C	Vitis vinifera

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Release 2023.1 (January 2023)