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Literature summary for 2.2.1.6 extracted from
- A new amino acid substitution (Ala-205-Phe) in acetolactate synthase (ALS) confers broad spectrum resistance to ALS-inhibiting herbicides (2016), Planta, 243, 149-159.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ALS, recombinant exxpression of N-terminally His6-tagged wild-type and mutant enzymes with a 85 amino acid N-terminal deletion eliminating the chloroplast transit peptide | Arabidopsis thaliana |
| gene ALSa, DNA and amino acid sequence determination and analysis, genotyping, expression analysis | Poa annua |
| gene ALSb, DNA and amino acid sequence determination and analysis, genotyping, expression analysis | Poa annua |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A205F | site-directed mutagenesis, the enzyme mutation confers resistance to imidazolinone, sulfonylurea, triazolopyrimidines, sulfonylaminocarbonyl triazolinones, and pyrimidinyl(thio)benzoate herbicides | Arabidopsis thaliana |
| A205F | the A205F substitution in acetolactate synthase, confirmed in a population of allotetraploid annual bluegrass, confers resistance to imidazolinone, sulfonylurea, triazolopyrimidines, sulfonylamino-carbonyl-triazolinones, and pyrimidinyl (thio) benzoate herbicides | Poa annua |
| A205F | the A205F substitution in acetolactate synthase, confirmed in a population of allotetraploid annual bluegrass, confers resistance to imidazolinone, sulfonylurea, triazolopyrimidines, sulfonylaminocarbonyl triazolinones, and pyrimidinyl(thio)benzoate herbicides | Poa annua |
| A205F | the mutation confers high resistance to imidazolinones, sulfonylureas, triazolopyrimidines, sulfonylamino-carbonyl-triazolinones, and pyrimidinyl (thio) benzoate herbicides | Poa annua |
| A205V | site-directed mutagenesis | Arabidopsis thaliana |
| A205V | the mutation confers resistance to imidazolinones, sulfonylureas, triazolopyrimidines, sulfonylamino-carbonyl-triazolinones, and pyrimidinyl (thio) benzoate herbicides | Poa annua |
| W574L | site-directed mutagenesis | Arabidopsis thaliana |
| W574L | the mutation confers resistance to imidazolinones, sulfonylureas, triazolopyrimidines, sulfonylamino-carbonyl-triazolinones, and pyrimidinyl (thio) benzoate herbicides | Poa annua |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| bensulfuron | - |
Arabidopsis thaliana |  |
| bensulfuron | - |
Poa annua | |
| bispyribac | - |
Arabidopsis thaliana | |
| bispyribac | - |
Poa annua | |
| florasulam | - |
Arabidopsis thaliana | |
| florasulam | - |
Poa annua | |
| flucarbazone | - |
Arabidopsis thaliana | |
| flucarbazone | - |
Poa annua | |
| foramsulfuron | - |
Arabidopsis thaliana | |
| foramsulfuron | - |
Poa annua | |
| imazamox | - |
Arabidopsis thaliana | |
| imazamox | - |
Poa annua | |
| imazapyr | - |
Arabidopsis thaliana | |
| imazapyr | - |
Poa annua | |
| imazaquin | - |
Arabidopsis thaliana | |
| imazaquin | - |
Poa annua | |
| mesosulfuron | - |
Arabidopsis thaliana | |
| mesosulfuron | - |
Poa annua | |
| metosulam | - |
Arabidopsis thaliana | |
| metosulam | - |
Poa annua | |
| nicosulfuron | - |
Arabidopsis thaliana | |
| nicosulfuron | - |
Poa annua | |
| primisulfuron | - |
Arabidopsis thaliana | |
| primisulfuron | - |
Poa annua | |
| propoxycarbazone | - |
Arabidopsis thaliana | |
| propoxycarbazone | - |
Poa annua | |
| prosulfuron | - |
Arabidopsis thaliana | |
| prosulfuron | - |
Poa annua | |
| pyriftalid | - |
Arabidopsis thaliana | |
| pyriftalid | - |
Poa annua | |
| pyrimidinyl-(thio) benzoates | - |
Arabidopsis thaliana | |
| pyrimidinyl-(thio) benzoates | - |
Poa annua | |
| pyrithiobac | - |
Arabidopsis thaliana | |
| pyrithiobac | - |
Poa annua | |
| pyroxsulam | - |
Arabidopsis thaliana | |
| pyroxsulam | - |
Poa annua | |
| sulfonylurea | - |
Poa annua | |
| thiencarbazone | - |
Arabidopsis thaliana | |
| thiencarbazone | - |
Poa annua | |
| triazolopyrimidines | - |
Poa annua | |
| trifloxysulfuron | - |
Arabidopsis thaliana | |
| trifloxysulfuron | - |
Poa annua | |
| tritosulfuron | - |
Arabidopsis thaliana | |
| tritosulfuron | - |
Poa annua | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Arabidopsis thaliana | |
| Mg2+ | required | Poa annua | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 pyruvate | Arabidopsis thaliana | - |
2-acetolactate + CO2 | - |
? | |
| 2 pyruvate | Poa annua | - |
2-acetolactate + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | P17597 | - |
- |
| Poa annua | - |
annual bluegrass | - |
| Poa annua | A0A0M3TGF7 | Alsb; population of allotetraploid annual bluegrass, POAANR3, selected from golf course turf in Tennessee, USA, two homeologous forms of the ALS gene, ALSa and ALSb | - |
| Poa annua | A0A0M4NDK0 | Alsa; population of allotetraploid annual bluegrass, POAANR3, selected from golf course turf in Tennessee, USA, two homeologous forms of the ALS gene, ALSa and ALSb | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Poa annua |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Poa annua | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 pyruvate | - |
Arabidopsis thaliana | 2-acetolactate + CO2 | - |
? | |
| 2 pyruvate | - |
Poa annua | 2-acetolactate + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ALS | - |
Arabidopsis thaliana |
| ALS | - |
Poa annua |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Arabidopsis thaliana |
| 30 | - |
assay at | Poa annua |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Arabidopsis thaliana |
| 7 | - |
assay at | Poa annua |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | required | Arabidopsis thaliana | |
| FAD | required | Poa annua | |
| thiamine diphosphate | - |
Poa annua | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.000000000441 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | metosulam | |
| 0.000000000742 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | pyrithiobac | |
| 0.00000000133 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | mesosulfuron | |
| 0.00000000158 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | bispyribac | |
| 0.00000000288 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | prosulfuron | |
| 0.00000000314 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | primisulfuron | |
| 0.00000000343 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | florasulam | |
| 0.00000000412 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | pyroxsulam | |
| 0.00000000423 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | bensulfuron | |
| 0.0000000059 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | trifloxysulfuron | |
| 0.0000000075 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | foramsulfuron | |
| 0.00000000928 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | propoxycarbazone | |
| 0.0000000187 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | tritosulfuron | |
| 0.0000000245 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | flucarbazone | |
| 0.0000000248 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | thiencarbazone | |
| 0.0000000714 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | nicosulfuron | |
| 0.000000441 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | metosulam | |
| 0.000000742 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | pyrithiobac | |
| 0.00000133 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | mesosulfuron | |
| 0.00000155 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | imazaquin | |
| 0.00000158 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | bispyribac | |
| 0.00000288 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | prosulfuron | |
| 0.00000293 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | imazamox | |
| 0.00000314 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | primisulfuron | |
| 0.00000343 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | florasulam | |
| 0.00000412 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | pyroxsulam | |
| 0.00000423 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | bensulfuron | |
| 0.0000059 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | trifloxysulfuron | |
| 0.00000606 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | imazapyr | |
| 0.0000075 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | foramsulfuron | |
| 0.0000187 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | tritosulfuron | |
| 0.00002 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | pyriftalid | |
| 0.0000245 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | flucarbazone | |
| 0.0000248 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | thiencarbazone | |
| 0.0000714 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | nicosulfuron | |
| 0.0000928 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | propoxycarbazone | |
| 0.00155 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | imazaquin | |
| 0.00293 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | imazamox | |
| 0.00606 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | imazapyr | |
| 200 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | pyriftalid | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | the A205F substitution in acetolactate synthase, confirmed in a population of allotetraploid annual bluegrass, confers resistance to imidazolinone, sulfonylurea, triazolopyrimidines, sulfonylaminocarbonyl triazolinones, and pyrimidinyl(thio)benzoate herbicides | Poa annua |
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