Literature summary for 2.2.1.6 extracted from

Pang, S.S.; Duggleby, R.G.
Expression, purification, characterization, and reconstitution of the large and small subunits of yeast acetohydroxyacid synthase (1999), Biochemistry, 38, 5222-5231.

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Inhibitors

Inhibitors	Comment	Organism	Structure
L-valine	not inhibitory for catalytic subunit alone, inhibitory for catalytic subunit plus small subunit	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	expression of both small and catalytic subunit in Escherichia coli	-

Renatured (Commentary)

Renatured (Comment)	Organism
reconstitution of enzyme from small and catalytic subunit each overexpressed in Escherichia coli and purified. Small subunit protein stimulates catalytic subunit up to 7fold and confers upon it sensitivity to inhibition by valine	Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
6.8	-	catalytic subunit, pH 7.0, 30°C	Saccharomyces cerevisiae
49	-	catalytic subunit plus small subunit, pH 7.0, 30°C	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pyruvate	-	Saccharomyces cerevisiae	2-acetolactate + CO2	-	?

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.16	-	L-valine	catalytic subunit plus small subunit, pH 7.0, 30°C	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)